YUCATAN MINIATURE SWINE AS A MODEL FOR DIET-INDUCED ATHEROSCLEROSIS
YUCATAN MINIATURE SWINE AS A MODEL FOR DIET-INDUCED ATHEROSCLEROSIS
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DOI：
10.1016/0021-9150(82)90104-6
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发表时间：
1982-01-01
期刊：
影响因子：
5.3
通讯作者：
FRY, DL
中科院分区：
文献类型：
--
作者：
REITMAN, JS;MAHLEY, RW;FRY, DL
9只雌性尤卡坦小型猪，一个以前没有评估其作为实验性动脉粥样硬化研究模型的潜在有用性的品种，被喂食高脂肪，高胆固醇饮食10-12个月。在此阶段结束时，对这些猪和4只对照（低脂、低胆固醇饲养）猪进行完整尸检，通过肉眼和显微镜检查表征动脉粥样硬化。胆固醇喂养导致血清胆固醇水平升高，加速动脉粥样硬化的发展。低胆固醇饮食的对照动物几乎没有肉眼或显微镜下的动脉粥样硬化。通过对苏丹染色的动脉组织进行肉眼检查，所有喂食胆固醇的猪都比任何对照组具有更广泛的动脉粥样硬化。存在个体差异，表明除血浆胆固醇外，其他因素的相互作用决定了动脉粥样硬化的程度和严重程度。高胆固醇血症与终末腹主动脉和肠系膜动脉内膜增厚以及肠系膜动脉脂肪沉积增加之间可能呈正相关。胆固醇诱导的动脉粥样硬化以脂质在细胞内和细胞周围沉积为特征。复杂的动脉粥样硬化病变与人类动脉粥样硬化相似，其特征是显著的内膜增生、坏死、胆固醇结晶沉积和钙化。
Nine female Yucatan miniature swine, a breed not previously evaluated for their potential usefulness as a model for experimental atherosclerosis studies, were fed a high-fat, high-cholesterol diet for 10-12 mo. These swine and 4 control (low-fat, low-cholesterol-fed) swine underwent a complete necropsy at the end of this period to characterize the atherosclerosis both by gross and microscopic examination. Cholesterol feeding led to elevated serum cholesterol levels and the development of accelerated atherosclerosis. Control animals on a low-cholesterol diet had little gross or microscopic atherosclerosis. All of the cholesterol-fed swine had more extensive atherosclerosis than any of the controls by gross inspection of the Sudan-stained arterial tissue. There was individual variation suggesting the interaction of factors in addition to the plasma cholesterol which determine the extent and severity of atherosclerosis. It was possible to show a positive correlation between hypercholesterolemia and intimal thickening in the terminal abdominal aorta and mesenteric artery and increased fat deposition in the mesenteric artery. The cholesterol-induced atherosclerosis was characterized by the deposition of lipid in and around cells. Complicated atherosclerotic lesions similar to human atherosclerosis were characterized by marked intimal proliferation, necrosis, cholesterol crystal deposition and calcification.