The decidual suction method: a new way of collecting decidual tissue for functional and morphological studies
The decidual suction method: a new way of collecting decidual tissue for functional and morphological studies
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DOI：
10.1111/j.0001-6349.2004.00395.x
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发表时间：
2004-08-01
影响因子：
4.3
通讯作者：
Roald, B
中科院分区：
文献类型：
--
作者：
Harsem, NK;Staff, AC;Roald, B
背景绒毛外滋养层细胞和胎盘床螺旋动脉的研究对于更好地理解病理性妊娠如先兆子痫、宫内生长受限和糖尿病是必不可少的。一个主要的挑战是获得有代表性的和足够的组织形态和功能的研究。目前，组织材料主要通过胎盘床活检（PBB）获得。我们描述了一种新的吸引方法，以获得更大体积的蜕膜组织从胎盘床。在51例剖宫产手术中，通过胎盘床的真空抽吸获得组织。将来自福尔马林固定的石蜡包埋的组织的切片常规地用苏木精和伊红（H&E）染色，用一组抗体免疫染色，并对滋养层细胞和螺旋动脉的存在进行形态学检查。结果与多溴联苯档案材料和胎盘基板切片（BPS）的结果进行了比较。记录了研究患者的短期不良事件。对151例接受过蜕膜抽吸法（DSM）的妇女的病历记录了长期并发症，随访38-60个月。在86%（n = 44）中，来自蜕膜抽吸材料的一个随机切片显示至少一个螺旋动脉。在37%（n = 19）中，存在六个或更多螺旋动脉。所有切片均显示绒毛外滋养细胞。没有短期或长期的发病率记录。蜕膜抽吸法是一项重要的改进，可以获得足够的蜕膜组织，用于绒毛外滋养层功能和螺旋动脉适应的形态和功能研究。该方法是安全的，因为没有短期或长期并发症。
Background. Studies of extravillous trophoblasts and placental bed spiral arteries are essential for a better understanding of pathological pregnancies such as preeclampsia, intrauterine growth restriction and diabetes mellitus. A major challenge is to obtain representative and sufficient tissue for morphological and functional investigations. Currently, tissue material is mostly harvested by placental bed biopsy (PBB). We describe a new suction method to obtain a larger volume of decidual tissue from the placental bed.Methods. Tissue was harvested in 51 cesarean sections by vacuum suction of the placental bed. Sections from formalin-fixed, paraffin-embedded tissue were routinely stained with hematoxylin and eosin (H&E), immunostained with a panel of antibodies and morphologically examined for the presence of trophoblasts and spiral arteries. The results were compared with those from archive material from PBBs and placental basal plate sections (BPSs). Short-term adverse events were registered for the study patients. Long-term complications were registered from medical charts of 151 women having undergone the decidual suction method (DSM), with a follow-up of 38-60 months.Results. In 86% (n = 44), one random section from the decidual suction material demonstrated at least one spiral artery. In 37% (n = 19), six or more spiral arteries were present. All sections revealed extravillous trophoblasts. No short- or long-term morbidity was recorded.Conclusions. The decidual suction method represents an important improvement in the work to obtain sufficient decidual tissue for morphological and functional studies of extravillous trophoblast function and spiral artery adaptation. The method is safe, as no short- or long-term complications were registered.