Lactobacillus bombi sp nov., from the digestive tract of laboratory-reared bumblebee queens (Bomb us terrestris)
Lactobacillus bombi sp nov., from the digestive tract of laboratory-reared bumblebee queens (Bomb us terrestris)
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DOI：
10.1099/ijs.0.063602-0
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发表时间：
2014-08-01
影响因子：
2.8
通讯作者：
Hroncova, Z.
中科院分区：
文献类型：
--
作者：
Killer, J.;Votavova, A.;Hroncova, Z.
在厌氧条件下，利用MRS琼脂从实验室饲养的大黄蜂（Bombus terrestris）的消化道中分离出三株乳杆菌属细菌。根据16S rRNA基因序列分析，分离菌株与未鉴定菌株Lactobacillus sp.的16S rRNA基因序列相似性最高（96.9%）。从欧洲蜜蜂（Apis mellifera）胃中分离的乳酸菌（Lactobacillus tucceti）的Mboho2r2是最近的有效已发表菌株，16S rRNA基因序列与该型菌株的相似性为92.9%。然而，基于不同标记的系统发育分析表明，该物种与新菌株的系统发育距离很远。菌株BTLCH M1/2(T)的DNA G+C含量为37.8 mol%。脂肪酸C-19:1 ω - 6c和/或C-19:0 cyclo ω - 10c/19 ω - 6、C-18:1 ω - 9C和C-16:0在所有菌株中均占主导地位。在新菌株中检测到二磷脂酰甘油、磷脂酰甘油、一种磷脂、七种糖脂和两种磷酸糖脂。47℃时观察生长情况。菌株BTLCH M1/2(T)的肽聚糖类型为A4 α L-Lys-D-Asp。基于系统发育标记hsp60、pheS、rpoA和tuf的基因型特征和系统发育分析以及化学分类分析结果证实该分离株属于乳杆菌属的一个新种，命名为乳杆菌bombi sp. 11。型应变为BTLCH M1/2(T) (=DSM 26517（T)=CCM 8440(T)）。
Three bacterial strains belonging to the genus Lactobacillus were isolated from the digestive tracts of laboratory-reared bumblebee queens (Bombus terrestris) using MRS agar under anaerobic conditions. The isolates were identified according to 16S rRNA gene sequence analysis as undescribed members of the genus Lactobacillus, with the highest 16S rRNA gene sequence similarity (96.9%) to the uncharacterized bacterial strain Lactobacillus sp. Mboho2r2 isolated from the stomach of a European honeybee (Apis mellifera Lactobacillus tucceti was found to be the closest related species with a validly published name, with 92.9% 16S rRNA gene sequence similarity to the type strain. However, phylogenetic analyses based on different markers revealed that this species is phylogenetically very distant from the novel strains. The DNA G+C content of the proposed type strain BTLCH M1/2(T) is 37.8 mol%. The fatty acids C-19:1 omega 6c and/or C-19:0 cyclo omega 10c/19 omega 6, C-18:1 omega 9C and C-16:0 were predominant in all strains. Diphosphatidylglycerol, phosphatidylglycerol, a phospholipid, seven glycolipids and two phosphoglycolipids were detected in the novel strains. Growth was observed at 47 C. The peptidoglycan type A4 alpha L-Lys-D-Asp was determined for strain BTLCH M1/2(T). Genotypic characteristics and phylogenetic analyses based on the phylogenetic markers hsp60, pheS, rpoA and tuf as well as phenotypic characteristics and the results of chemotaxonomic analyses confirmed that the new isolates belong to a novel species of the genus Lactobacillus, for which the name Lactobacillus bombi sp. nov. is proposed. The type strain is BTLCH M1/2(T) (=DSM 26517(T)=CCM 8440(T)).