Immunoadsorption of Cryptococcus-specific suppressor T-cell factors.
Immunoadsorption of Cryptococcus-specific suppressor T-cell factors.
复制标题
隐球菌特异性抑制性 T 细胞因子的免疫吸附。
DOI：
10.1128/iai.51.3.844-850.1986
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发表时间：
1986
影响因子：
3.1
通讯作者：
Cox,RA
中科院分区：
文献类型：
--
作者：
Mosley,RL;Murphy,JW;Cox,RA
在鼠隐球菌抑制细胞回路中，已经鉴定了两种不同的T细胞抑制因子TsF 1和TsF 2，其特异性抑制对隐球菌培养滤液抗原（CneF）的迟发型超敏反应（DTH）。TsF 1由一级T抑制细胞（Ts 1）产生，抑制DTH反应的传入肢，而TsF 2由二级T抑制细胞（Ts 2）产生，抑制隐球菌DTH反应的传出肢。本研究的目的是确定是否TsF 1或TsF 2可以结合隐球菌抗原。为了评估这一点，用热灭活的新型隐球菌细胞和通过固相免疫吸附（SPIA）在含有隐球菌抗原的柱上进行TsF 1和TsF 2的吸附，即，CneF与Sepharose 4 B共价结合。TsF 1的抑制作用通过用完整的热灭活的隐球菌吸附和通过CneF-Sepharose 4 B上的SPIA去除。隐球菌TsF 1的隐球菌SPIA柱的结合被证明是特异性的，因为Sepharose 4 B柱与酿酒酵母甘露聚糖偶联或用甘氨酸封闭不吸附抑制剂活性。与此相反，TsF 2的抑制组分不与热灭活的隐球菌CneF-Sepharose 4 B、S.酿酒酵母甘露聚糖-琼脂糖4 B或甘氨酸-琼脂糖4 B柱。这些结果，连同发现隐球菌抗原，抗隐球菌抗体，C1 q结合免疫复合物没有表现出在任何TsF 1或TsF 2，建立TsF 1和TsF 2可以区分的基础上，他们的隐球菌抗原的亲和力。
In the murine cryptococcal suppressor cell circuit, two different T-cell suppressor factors, TsF1 and TsF2, have been identified which specifically suppress the delayed-type hypersensitivity (DTH) response to cryptococcal culture filtrate antigen (CneF). TsF1 is produced by a first-order T suppressor (Ts1) cell population and suppresses the afferent limb of the DTH response, whereas TsF2 is produced by a second-order T suppressor (Ts2) cell population and suppresses the efferent limb of the cryptococcal DTH response. The objective of this study was to ascertain whether TsF1 or TsF2 could bind to cryptococcal antigen. To assess this, adsorption of TsF1 and TsF2 was performed with heat-killed Cryptococcus neoformans cells and by solid-phase immunoadsorption (SPIA) on columns containing cryptococcal antigens, i.e., CneF covalently bound to Sepharose 4B. The suppressive effect of TsF1 was removed by adsorption with intact heat-killed cryptococci and by SPIA on CneF-Sepharose 4B. The binding of cryptococcal TsF1 to the cryptococcal SPIA column was shown to be specific since Sepharose 4B columns either coupled with Saccharomyces cerevisiae mannan or blocked with glycine did not adsorb the suppressor activity. In contrast, the suppressive component of TsF2 did not bind to heat-killed cryptococci, CneF-Sepharose 4B, S. cerevisiae mannan-Sepharose 4B, or glycine-Sepharose 4B columns. These results, together with the finding that cryptococcal antigen, anticryptococcal antibody, and C1q-binding immune complexes were not demonstrated in either TsF1 or TsF2, establish that TsF1 and TsF2 can be differentiated on the basis of their affinity for cryptococcal antigen.