Histamine-induced calcium mobilization in single cultured cells expressing histamine H1 receptors: a relationship between its sensitivity and the density of H1 receptors.
Histamine-induced calcium mobilization in single cultured cells expressing histamine H1 receptors: a relationship between its sensitivity and the density of H1 receptors.
复制标题
表达组胺 H1 受体的单个培养细胞中组胺诱导的钙动员：其敏感性与 H1 受体密度之间的关系。
DOI：
10.3892/ijmm.1.2.355
复制
发表时间：
1998
影响因子：
5.4
通讯作者：
T. Watanabe
中科院分区：
文献类型：
--
作者：
Y. Ohuchi;K. Yanai;E. Sakurai;H. Fukui;T. Yanagisawa;T. Watanabe
组胺H1受体的刺激启动磷脂酰肌醇的水解，并导致肌醇（1，4，5）-三磷酸的产生和细胞内Ca 2+动员。虽然通过H1受体的信号转导机制已被广泛研究，很少有人知道组胺诱导的Ca 2+动员的敏感性和H1受体在培养细胞中的密度之间的相关性。用Ca 2+敏感染料Indo-1和动态单细胞成像技术（ACAS 570激光细胞仪）监测组胺刺激后单个CHO细胞和稳定表达H1受体的大鼠C6-胶质瘤细胞以及星形细胞瘤1321 N1细胞的胞浆游离Ca 2+浓度（[Ca 2 +]i）。表达H1受体的CHO细胞和C6神经胶质瘤细胞对组胺的敏感性比天然存在H1受体的星形细胞瘤1321 N1细胞高10倍以上。转染细胞中H1受体的密度也是1321 N1细胞的10倍以上。此外，毒胡萝卜素抑制细胞内Ca ~（2+）-ATP酶可引起H_1受体过表达细胞和星形细胞瘤1321N_1细胞内[Ca ~（2+）]i增加，其敏感性相似。这些数据表明，在这些细胞中组胺的Ca ~（2+）动员的敏感性与H_1受体密度的增加相关增强。
Stimulation of histamine H1 receptors initiates the hydrolysis of phosphatidylinositides and results in the production of inositol (1, 4,5)-triphosphate and intracellular Ca2+ mobilization. Although the mechanism for signal transduction via the H1 recptor has been extensively investigated, little is known about the correlation between the sensitivity of histamine-induced Ca2+ mobilization and the density of H1 receptors in cultured cells. Cytosolic free Ca2+ concentration ([Ca2+]i) after stimulation by histamine was monitored in single CHO and rat C6-glioma cells stably expressed with H1 receptors and astrocytoma 1321N1 cells using the Ca2+-sensitive dye Indo-1 and dynamic single cell imaging techniques (ACAS 570 laser cytometer). Both of the H1 receptor-expressed CHO cells and C6-glioma cells were over 10 times more sensitive to histamine than astrocytoma 1321N1 cells in which H1 receptors were naturally present. The density of H1 receptors in the transfected cells was also more than 10-fold that of 1321N1 cells. In addition, inhibition of intracellular Ca2+-ATPase by thapsigargin elicited an increase in [Ca2+]i in H1 receptor-overexpessed cells and astrocytoma 1321N1 cells with similar sensitivity. These data suggest that the sensitivity of Ca2+ mobilization by histamine in these cells was correlatively augmented with the increase in the density of H1 receptors.