IDENTIFICATION AND CHARACTERIZATION OF A REGULATED PROMOTER ELEMENT IN THE EPIDERMAL GROWTH-FACTOR RECEPTOR GENE
IDENTIFICATION AND CHARACTERIZATION OF A REGULATED PROMOTER ELEMENT IN THE EPIDERMAL GROWTH-FACTOR RECEPTOR GENE
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DOI：
10.1073/pnas.87.19.7536
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发表时间：
1990-10-01
影响因子：
11.1
通讯作者：
GILL, GN
中科院分区：
文献类型：
--
作者：
HUDSON, LG;THOMPSON, KL;GILL, GN
我们已经在表皮生长因子受体5“区域中鉴定出一个36碱基对的近端元件（相对于AUG翻译起始密码子为-112至-77），其功能为启动子;介导对表皮生长因子、佛波醇12-肉豆蔻酸酯13-乙酸酯和环AMP的诱导反应;并以不依赖于方向的方式起作用。当转移到含有TATA盒的异源启动子时，该区域起增强子的作用。36个碱基对区域内的突变改变受体细胞中报告基因表达测定的功能。已经鉴定了一种蛋白质，该蛋白质对与体内观察到的启动子活性相关的突变体DNA序列表现出适当的结合特异性。基于DNA结合特征和大小，所鉴定的蛋白质似乎与已知结合到富含G+ C的区域的几种先前鉴定的转录因子不同。
We have identified a 36-base-pair proximal element (-112 to -77 relative to the AUG translation initiation codon) in the epidermal growth factor receptor 5'' region that functions as a promoter; mediates inductive responses to epidermal growth factor, phorbol 12-myristate 13-acetate, and cyclic AMP; and acts in an orientation-independent manner. This region functions as an enhancer when transferred to a heterologous promoter containing a TATA box. Mutations within the 36-base-pair region alter function as assayed by reporter gene expression in recipient cells. A protein has been identified that demonstrates appropriate binding specificity to mutant DNA sequences that correlates with promoter activity observed in vivo. On the basis of DNA binding characteristics and size, the identified protein appears distinct from several previously identified transcription factors known to bind to G+C-rich regions.