A comparative study on the leaf anatomical structure of Camellia oleifera in a low-hot valley area in Guizhou Province, China.
A comparative study on the leaf anatomical structure of Camellia oleifera in a low-hot valley area in Guizhou Province, China.
复制标题
贵州省低热河谷地区油茶叶片解剖结构比较研究
DOI：
10.1371/journal.pone.0262509
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Hu Y;Yang L;Gao C;Liao D;Long L;Qiu J;Wei H;Deng Q;Zhou Y
叶片是植物重要的同化器官，叶片的解剖结构在一定程度上可以反映植物对环境的适应性。为培育上级地方品种，从油茶种质资源圃中选取35株健康单株，对其叶片结构进行比较研究。2019年7月，从35个选择的健康C.用石蜡切片法对油茶植株的叶片结构进行了观察。利用方差分析、相关分析和聚类分析筛选健康单株。根据聚类隶属度、相关指数和变异系数（C/V）选取代表性指标，通过隶属函数对抗旱性进行综合评价。35株健康植株叶片结构的11项指标差异极显著。C18叶片厚度最大，C7海绵组织最大，C38栅栏组织厚度与海绵组织厚度之比（P/S）最大。健康植株的聚类结果差异显著。隶属函数分析表明，35 C.将油茶植物分为五类。C18具有很强的抗旱性，C3、C7和C40具有较强的抗旱性。结果表明，不同品种间在上表皮、P/S比值、海绵组织等方面存在显著差异。油茶属植物C18、C3、C7和C40表现出较好的抗旱性。C39和C26抗旱性中等，但P/S值较高，可用于培育高产、抗旱的玉米品种。油茶品种叶片P/S比随叶龄的增加而增加。低热河谷地区的油茶产量较高。在各种叶结构中，海绵组织、上表皮、P/S比和角质层是评价毛白杨抗旱性的指标。生长在低热山谷地区的一种油茶。
The leaf serves as an important assimilation organ of plants, and the anatomical structure of leaves can reflect the adaptability of the plant to the environment to a certain extent. The current study aimed to cultivate superior local cultivars, and 35 healthy individual plants were selected from the Camellia oleifera germplasm resource nursery for a comparative study of the leaf structure. In July 2019, the leaves were collected from 35 selected healthy C. oleifera plants, and the leaf structure was observed by using the paraffin section method. Healthy individual plants were screened using variance analysis, correlation analysis and cluster analysis. The representative indices were selected according to the cluster membership, correlation indices and coefficient of variation (C/V) for a comprehensive evaluation of drought resistance via the membership function. There were extremely significant differences in 11 indices of leaf structure for these 35 healthy plants. C18 had the greatest leaf thickness, C7 the largest spongy tissue, and C38 the largest ratio of palisade tissue thickness to spongy tissue thickness (P/S). The clustering results of the healthy individual plants differed significantly. The membership function showed that the drought resistance of 35 C. oleifera plants was divided into five categories. C18 had very strong drought resistance, and C3, C7 and C40 had strong drought resistance. There were significant differences in terms of the upper epidermis, P/S ratio and spongy tissue among the C. oleifera plants. C18, C3, C7 and C40 exhibited satisfactory drought resistance. Although C39 and C26 had moderate drought resistance, their P/S ratios were high, which might be used to cultivate high-yield and drought-resistant C. oleifera varieties. The leaf P/S ratio of C. oleifera from low-hot valley areas was high. Among various leaf structures, spongy tissue, upper epidermis, P/S ratio and cuticle constitute the drought resistance evaluation indices for C. oleifera grown in low-hot valley areas.