N-methyl mesoporphyrin IX as a highly selective light-up probe for G-quadruplex DNA.
N-methyl mesoporphyrin IX as a highly selective light-up probe for G-quadruplex DNA.
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DOI：
10.1142/s1088424619300179
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发表时间：
2019-12
影响因子：
1.5
通讯作者：
Yatsunyk LA
中科院分区：
文献类型：
--
作者：
Yett A;Lin LY;Beseiso D;Miao J;Yatsunyk LA
N-甲基中卟啉IX（NMM）是一种水溶性的非对称卟啉，具有优异的光学性质和对G-四链体（GQ）DNA无与伦比的选择性。G-四链体是由富含鸟嘌呤的序列形成的非规范DNA结构。它们与基因组稳定性、寿命和癌症有关。NMM选择性识别GQ结构的能力使其成为设计新型GQ结合剂的有价值的支架。在这篇综述中，我们调查了文献描述的GQ结合特性的NMM以及其在化学和生物学的广泛用途。我们从NMM的GQ结合特性的发现和NMM结合适体的开发开始。然后我们讨论了NMM的光学性质，重点是光开关效应-NMM与GQ DNA结合后诱导的高荧光。此外，我们研究了NMM对GQ的亲和力和选择性，以及其稳定GQ结构和有利于平行GQ构象的能力。此外，一部分的审查是专门的应用NMM-GQ配合物作为重金属，小分子（如ATP和农药），DNA和蛋白质的生物传感器。最后，重要的是，我们讨论了实用的NMM作为探针，调查GQs在生物过程中的作用。
N-methyl mesoporphyrin IX (NMM) is a water-soluble, non-symmetric porphyrin with excellent optical properties and unparalleled selectivity for G-quadruplex (GQ) DNA. G-quadruplexes are non-canonical DNA structures formed by guanine-rich sequences. They are implicated in genomic stability, longevity, and cancer. The ability of NMM to selectively recognize GQ structures makes it a valuable scaffold for designing novel GQ binders. In this review, we survey the literature describing the GQ-binding properties of NMM as well as its wide utility in chemistry and biology. We start with the discovery of the GQ-binding properties of NMM and the development of NMM-binding aptamers. We then discuss the optical properties of NMM, focusing on the light-switch effect — high fluorescence of NMM induced upon its binding to GQ DNA. Additionally, we examine the affinity and selectivity of NMM for GQs, as well as its ability to stabilize GQ structures and favor parallel GQ conformations. Furthermore, a portion of the review is dedicated to the applications of NMM-GQ complexes as biosensors for heavy metals, small molecules (e.g. ATP and pesticides), DNA, and proteins. Finally and importantly, we discuss the utility of NMM as a probe to investigate the roles of GQs in biological processes.
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DOI：
10.2174/1568011054222292
发表时间：
2005-07-01
期刊：
Current Medicinal Chemistry - Anti-Cancer Agents
影响因子：
--
作者：
Chaires, Jonathan B.
通讯作者：
Chaires, Jonathan B.
影响因子：
7.4
作者：
BEALE, SI;CHEN, NC
通讯作者：
CHEN, NC
影响因子：
6.1
作者：
Chen, Piaopiao;Huang, Ke;Geng, Jia
通讯作者：
Geng, Jia
影响因子：
6.1
作者：
Guo, Yahui;Xu, Pei;Hu, Jiming
通讯作者：
Hu, Jiming
影响因子：
14.9
作者：
Beaume N;Pathak R;Yadav VK;Kota S;Misra HS;Gautam HK;Chowdhury S
通讯作者：
Chowdhury S