SPECIFIC EXPRESSION OF THE PANCREATIC-SECRETORY-TRYPSIN-INHIBITOR (PSTI) GENE IN HEPATOCELLULAR-CARCINOMA
SPECIFIC EXPRESSION OF THE PANCREATIC-SECRETORY-TRYPSIN-INHIBITOR (PSTI) GENE IN HEPATOCELLULAR-CARCINOMA
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DOI：
10.1002/ijc.2910550505
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发表时间：
1993-11-11
影响因子：
6.4
通讯作者：
MATSUBARA, K
中科院分区：
文献类型：
--
作者：
OHMACHI, Y;MURATA, A;MATSUBARA, K
应用北方印迹法检测20例肝细胞癌（HCC）胰腺分泌型胰蛋白酶抑制剂（PSTI）的表达。该基因在所有HCC中表达，但在其他肿瘤中不表达，包括乳腺癌、甲状腺癌、肺癌和卵巢癌。部分胃癌和结肠癌弱表达PSTI。在以类似方式检查的细胞系中，PSTI在所有4个来自肝癌的细胞系中表达。另一方面，在15个来源于肝癌以外的癌症的细胞系中，仅3个来源于胰腺癌、结肠癌和胃癌的细胞系弱表达PSTI。使用来自克隆的PSTI基因的5'区的缺失组的CAT测定已经显示，在肝细胞瘤细胞系中，该基因的表达依赖于包括IL-6应答元件和AP-1结合位点的2个调节区的存在。然而，在非肝癌细胞系中，这2个调控区不是表达所必需的。27例HCC患者血中PSTI水平显著升高，且与肿瘤大小呈正相关，提示PSTI在HCC中特异性表达导致这种效应，无炎症时血中PSTI水平升高提示HCC的存在。(C)1993 Wiley-Liss，Inc.
Twenty hepatocellular carcinomas (HCC) were analyzed by Northern blotting to test the expression of pancreatic secretory trypsin inhibitor (PSTI). This gene was expressed in all HCCs, but not in other tumors, including mammary, thyroid, pulmonary and ovarian cancers. Some gastric and colonic cancers weakly expressed PSTI. Among cell lines examined in a similar manner, PSTI was expressed in all of 4 derived from hepatoma. On the other hand, among 15 cell lines derived from cancers other than hepatoma, only 3, derived from pancreatic, colonic and gastric cancers, weakly expressed PSTI. A CAT assay using a deletion set of the 5' region from the cloned PSTI gene has shown that in hepatoma cell lines, the expression of this gene is dependent on the presence of 2 regulatory regions that include an IL-6 responsive element and an AP-I-binding site. However, in non-hepatoma cell lines, the 2 regulatory regions are not necessary for expression. The blood level of PSTI in 27 patients with HCC was significantly increased, and it was positively correlated with tumor size, suggesting that specific expression of PSTI in HCC causes this effect and that elevated blood level of PSTI without inflammation indicates the presence of HCC. (C) 1993 Wiley-Liss, Inc.