Genetic analysis of disease resistance to Vibrio harveyi by challenge test in Chinese tongue sole (Cynoglossus semilaevis)
Genetic analysis of disease resistance to Vibrio harveyi by challenge test in Chinese tongue sole (Cynoglossus semilaevis)
复制标题
半滑舌鳎 (Cynoglossus semilaevis) 哈维氏弧菌抗病性遗传分析
DOI：
10.1016/j.aquaculture.2019.01.011
复制
发表时间：
2019-03-30
期刊：
影响因子：
4.5
通讯作者：
Chen, Songlin
中科院分区：
文献类型：
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作者：
Li, Yangzhen;Wang, Lei;Chen, Songlin
近期，哈维氏弧菌已成为一种重要的致病病菌，在中国舌鳎中具有较高的死亡率（50% - 70%）。本研究的目的是评估中国舌鳎对哈维氏弧菌抗性的遗传参数，并评估抗性与生长性状（即体重和全长）之间的遗传相关性（涉及50个全同胞家系和8547个个体）。通过使用不同的性状定义（二分类和多分类）拟合纵向线性模型和横断面阈值模型。经过14天的试验，总体攻毒试验存活率为57.86%（家系存活率范围从9.30%到94.30%）。存活率的遗传力范围从0.11到0.28，线性模型得到的估计值高于阈值模型。抗性（二分类和多分类性状）与两种生长性状之间的遗传相关性呈中等正相关（0.27 - 0.51）。不同模型之间全同胞家系估计育种值的皮尔逊相关系数和斯皮尔曼相关系数都非常高（0.99到1），都接近1，这可能表明这四种模型具有相似的预测能力。有利的遗传力和中等正相关的遗传相关性表明，对弧菌病抗性和生长性能进行联合遗传改良是可行的。相关性说明：本文为中国舌鳎的抗病选育策略提供了指导。
Recently, Vibrio harveyi has emerged as an important pathogenic disease with a high mortality rate (50%-70%) in Chinese tongue sole. The aim of this study was to estimate genetic parameters for resistance to V. harveyi in Chinese tongue sole and to evaluate the genetic correlation between resistance and growth traits (i.e. body weight and total length) (involving 50 full-sib families and 8547 individuals). Longitudinal linear models and cross-sectional threshold models were fitted by using different trait definitions (binary and categorical). After a 14-day test, the overall challenge test survival was 57.86% (ranging from 9.30% to 94.30% in families). The heritabilities of survival were ranging from 0.11 to 0.28, estimates obtained by linear models were higher than threshold models. The genetic correlations between resistance (binary and categorical traits) and two growth traits were moderately positive (0.27-0.51). Very high Pearson and Spearman correlations (0.99 to 1) of full-sib family EBVs between different models were all close to unity which might reveal the similar predictive ability of the four models. The favourable heritabilities and moderate positive genetic correlations indicate that joint genetic improvement of vibriosis resistance and growth performance would be feasible.Statement of relevance: This paper offers guidelines to disease-resistance selective breeding strategies in Chinese tongue sole.