Genetic Recombination at the Buff Spore Color Locus in SORDARIA BREVICOLLIS. II. Analysis of Flanking Marker Behavior in Crosses between Buff Mutants.
Genetic Recombination at the Buff Spore Color Locus in SORDARIA BREVICOLLIS. II. Analysis of Flanking Marker Behavior in Crosses between Buff Mutants.
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SORDARIA BREVICOLLIS 浅黄色孢子颜色位点的基因重组。
DOI：
10.1093/genetics/103.2.161
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发表时间：
1983
期刊：
影响因子：
3.3
通讯作者：
H. Whitehouse
中科院分区：
文献类型：
--
作者：
H. Sang;H. Whitehouse
在侧链标记存在的情况下，从浅黄色位点等位基因对间杂交中筛选出含有一个或多个野生型孢子的变异子囊。获得了与异常分离位点和任何相关交叉位点有关的数据，从而对侧翼标记进行重组。在buff基因的近端位置的异常分离可能与异常分离位点近端的交叉有关，或者以相同的频率与在杂交中存在的第二个突变位点远端的交叉有关。类似地，远端位点的分离可能与远端位点的交叉有关，或者以较低的频率与近端突变位点的交叉有关。等位基因之间的交叉是罕见的。这一证据的杂交DNA和杂交之间的关系，讨论了目前的模型的重组机制。
Aberrant asci containing one or more wild-type spores were selected from crosses between pairs of alleles of the buff locus in the presence of closely linked flanking markers. Data were obtained relating to the site of aberrant segregation and the position of any associated crossover giving recombination of flanking markers. Aberrant segregation at a proximal site within the buff gene may be associated with a crossover proximal to the site of aberrant segregation or, with equal frequency, with a crossover distal to the site of the second mutant present in the cross. Similarly, segregation at a distal site may be associated with a crossover distal to the site or, with lower frequency, with a crossover proximal to the site of the proximal mutant present in the cross. Crossovers between the alleles were rare. This evidence for the relationship between hybrid DNA and crossing over is discussed in terms of current models for the mechanism of recombination.