A phenothiazine coumarin-based red emitting fluorescent probe for nanomolar detection of thiophenol with a large Stokes shift
A phenothiazine coumarin-based red emitting fluorescent probe for nanomolar detection of thiophenol with a large Stokes shift
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DOI：
10.1016/j.snb.2017.01.167
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发表时间：
2017-06-01
影响因子：
8.4
通讯作者：
Song, Xiangzhi
中科院分区：
文献类型：
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作者：
Chen, Wenqiang;Yue, Xiuxiu;Song, Xiangzhi
我们通过引入吩噻嗪骨架作为给电子组分，研制出一种独特的深红色功能性荧光染料5b，具有优异的光物理性能。以染料5b为平台，基于独特的噻吩介导的取代-环化-水解反应级联，我们进一步构建了一种新型的红色荧光探针(1)。探针1对硫酚的选择性和灵敏度高于脂肪族硫醇，检出限为2.9 nM （S/N = 3）。此外，该探针在PB缓冲液（pH= 7.4，含1 mM CTAB）中具有显著的大Stokes位移（141 nm）。重要的是，该探针已成功应用于实际水样和活HeLa细胞中噻吩的检测，表明该探针具有很大的实际应用潜力。(C) 2017 Elsevier B.V.版权所有
By introducing phenothiazine framework as an electron donating component, we have developed a unique deep-red functional fluorescent dye 5b, bearing excellent photophysical properties. Using dye 5b as a platform, we further constructed a novel red emitting fluorescent probe (1) for thiophenol on the basis of a unique thiophenol-mediated substitution-cyclization-hydrolysis reaction cascade. Probe 1 exhibits highly selective and sensitive response to thiophenol over aliphatic thiols, with a detection limit of 2.9 nM (S/N = 3). Moreover, this probe features a remarkable large Stokes shift (141 nm) in PB buffer (pH= 7.4, containing 1 mM CTAB). Importantly, it was successfully applied for detection of thiophenol both in real water samples and in living HeLa cells, indicating that this probe has great potential for practical applications. (C) 2017 Elsevier B.V. All rights reserved.