Metallic striped nanowires as multiplexed immunoassay platforms for pathogen detection
Metallic striped nanowires as multiplexed immunoassay platforms for pathogen detection
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DOI：
10.1002/anie.200601104
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发表时间：
2006-01-01
影响因子：
16.6
通讯作者：
Dougherty, George M.
中科院分区：
文献类型：
--
作者：
Tok, Jeffrey B. -H.;Chuang, Frank Y. S.;Dougherty, George M.
生物检测技术的迅速增长和发展主要是由于新的致命传染病的出现以及生物战成为新的恐怖主义手段。[1，2]为了满足便携式多重生物检测系统的需求，我们在此报告了一种新型生物传感平台，该平台使用工程化纳米线作为夹心免疫测定的替代基底（图1A）。纳米线是通过在多孔氧化铝模板内电沉积不同金属的亚微米层来构建的。[3，4]可以沉积多种金属：在这项研究中，我们采用了金、银和镍的条纹。由于排列中的金属可以被沉积，大量的独特的但容易识别的编码纳米线可以被包括在一个多路复用阵列格式。图像处理的光学反射图像可以使条纹图案被迅速识别，而荧光图像报告的信息之间的结合程度的抗体缀合的纳米线和荧光团标记的抗原靶。这种纳米线已被用于有效地检测和报告DNA杂交和免疫测定过程。[5，6]在此，我们证明了使用悬浮形式的多条纹金属纳米线（图1A）的可行性，以实现对生物战剂模拟物的快速和灵敏的单一和多重免疫测定。
The rapid growth and development in biodetection technology has largely been driven by the emergence of new and deadly infectious diseases and the realization of biological warfare as new means of terrorism.[1, 2] To address the need for portable, multiplex biodetection systems, we report here a novel biosensing platform using engineered nanowires as an alternative substrate for sandwich immunoassays (Figure 1A). The nanowires are built through submicrometer layering of different metals by electrodeposition within a porous alumina template.[3, 4] A variety of metals can be deposited: in this study, we employed stripes of gold, silver, and nickel. Owing to the permutations in which the metals can be deposited, a large number of unique yet easily identifiable encoded nanowires can be included in a multiplex array format.Image processing of an optical reflectance image can enable the stripe pattern to be identified rapidly, while fluorescence images report information on the degree of binding between the antibody-conjugated nanowires and a fluorophore-tagged antigen target. Such nanowires have been utilized to efficiently detect and report both DNA hybridization and immunoassay processes.[5, 6] Herein, we demonstrate the feasibility of using multistriped metallic nanowires (Figure1A) in a suspended format to enable rapid and sensitive single and multiplex immunoassays for biowarfare agent simulants.