PROPOSED PATHWAY FOR BIOSYNTHESIS OF THE S-LAYER GLYCOPROTEIN OF BACILLUS-ALVEI
PROPOSED PATHWAY FOR BIOSYNTHESIS OF THE S-LAYER GLYCOPROTEIN OF BACILLUS-ALVEI
复制标题
DOI：
10.1128/jb.175.14.4515-4519.1993
复制
发表时间：
1993-07-01
影响因子：
3.2
通讯作者：
KONIG, H
中科院分区：
文献类型：
--
作者：
HARTMANN, E;MESSNER, P;KONIG, H
真细菌肺芽孢杆菌的胞壁球囊的外表面被表面层（S 层）糖蛋白覆盖。该 S 层糖蛋白的聚糖链由以 ManNAc、Gal 和 Glc 作为成分的三糖重复单元组成。从肺芽胞杆菌的细胞提取物中，分离出 ManNAc、Gal、Glc 和 GlcNAc 的核苷酸激活衍生物。此外，还获得了GDP-和多荆基-活化的寡糖。在分离的假定糖蛋白前体的基础上，提出了寡糖链生物合成的途径。
The outer surface of the murein sacculus of the eubacterium Bacillus alvei is covered by a surface layer (S-layer) glycoprotein. The glycan chain of this S-layer glycoprotein consists of trisaccharide repeating units with ManNAc, Gal, and Glc as constituents. From cell extracts of B. alvei, nucleotide-activated derivatives of ManNAc, Gal, Glc, and GlcNAc were isolated. Furthermore, GDP- and dolichyl-activated oligosaccharides were obtained. On the basis of the isolated putative glycoprotein precursors, a pathway for the biosynthesis of the oligosaccharide chain is proposed.