Using DNA Metabarcoding to Identify Floral Visitation by Pollinators
Using DNA Metabarcoding to Identify Floral Visitation by Pollinators
复制标题
使用 DNA 元条形码识别传粉媒介对花朵的造访
DOI：
10.20944/preprints202202.0018.v1
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Natasha de Vere
中科院分区：
文献类型：
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作者：
Abigail Lowe;Laura Jones;L. Witter;S. Creer;Natasha de Vere
对传粉昆虫访花过程的研究为进一步了解植物与传粉昆虫之间的精细生态相互作用提供了机会，有助于保护生物多样性和促进生态系统健康。本文综述了用于鉴定花的各种方法，并对分子方法和非分子方法进行了比较。我们回顾了DNA元条形码在回答传粉者利用植物的生态问题方面的研究方法，并讨论了这项研究的结果。我们对元条形码工作流程的每一步都提出了详细的方法学考虑，从采样到扩增，最后是生物信息学分析。为研究人员使用这些技术提供了详细的指导，强调了方法标准化和提高结果可靠性的重要性。最后讨论了利用分子方法分析植物与传粉者相互作用的机会和方向。
The identification of floral visitation by pollinators provides an opportunity to improve our understanding of the fine-scale ecological interactions between plants and pollinators, contributing to biodiversity conservation and promoting ecosystem health. In this review we outline the various methods which can be used to identify floral visitation, providing a comparison between molecular and non-molecular methods. We review the literature covering the ways in which DNA metabarcoding has been used to answer ecological questions relating to plant use by pollinators and discuss the findings of this research. We present detailed methodological considerations for each step of the metabarcoding workflow, from sampling through to amplification and finally bioinformatic analysis. Detailed guidance is provided to researchers for utilization of these techniques, emphasizing the importance of standardization of methods and improving the reliability of results. Future opportunities and directions of using molecular methods to analyze plant-pollinator interactions are then discussed.
影响因子：
5.7
作者：
Bertrand, Colette;Eckerter, Philipp W.;Albrecht, Matthias
通讯作者：
Albrecht, Matthias
将巨幼蜂的花粉觅食与其巢细菌微生物群联系起来
影响因子：
2.6
作者：
Voulgari-Kokota A;Ankenbrand M;Grimmer G;Steffan-Dewenter I;Keller A.
通讯作者：
Keller A.
影响因子：
3
作者：
Dew, Rebecca M.;McFrederick, Quinn S.;Rehan, Sandra M.
通讯作者：
Rehan, Sandra M.