High-throughput microarray for antimicrobial susceptibility testing.
High-throughput microarray for antimicrobial susceptibility testing.
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用于抗菌药物敏感性测试的高通量微阵列。
DOI：
10.1016/j.btre.2017.10.004
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Ramasubramanian,AnandK
中科院分区：
文献类型：
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作者：
Srinivasan,Anand;Lee,GraceC;Torres,NelsonS;Hernandez,Kevin;Dallas,StevenD;Lopez-Ribot,Jose;Frei,ChristopherR;Ramasubramanian,AnandK
我们描述了一种新型，高通量，纳米级微阵列平台的发展，用于抗菌药物敏感性测试（AST）。该平台允许在单个芯片上以50 nL的体积处理480个样品，通过荧光读出简单的浸泡和冲洗步骤进行分析，并且能够同时处理多个样品和芯片。我们证明了该芯片在培养社区获得性耐甲氧西林金黄色葡萄球菌（CA-MRSA）方面的适用性，并对CA-MRSA临床分离株进行了AST检测。该芯片平台有望对微生物生物技术产生影响，作为传统的AST（如传统的肉汤微稀释或较新的自动化系统）的有吸引力的高通量、低样本量和更快的替代方案。
We describe the development of a novel, high-throughput, nano-scale microarray platform for antimicrobial susceptibility testing (AST). The platform allows to process 480 samples at 50 nL volume on a single chip, analyze by fluorescence read-out with an easy dunk-and-rinse step, and the ability to process multiple samples and chips simultaneously. We demonstrate the applicability of this chip for culturing community acquired methicillin resistantStaphylococcus aureus(CA-MRSA), and perform AST against clinical isolates of CA-MRSA. The chip platform holds promise for an impact in microbial biotechnology as an attractive high-throughput, lower sample volume and quicker alternative to conventional AST such as the traditional broth microdilution or the newer automated systems.