The Arabidopsis HINKEL gene encodes a kinesin-related protein involved in cytokinesis and is expressed in a cell cycle-dependent manner
The Arabidopsis HINKEL gene encodes a kinesin-related protein involved in cytokinesis and is expressed in a cell cycle-dependent manner
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DOI：
10.1016/s0960-9822(01)00655-8
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发表时间：
2002-01-22
期刊：
影响因子：
9.2
通讯作者：
Mayer, U
中科院分区：
文献类型：
--
作者：
Strompen, G;El Kasmi, F;Mayer, U
植物的细胞分裂始于分裂平面的中心，囊泡融合产生新的膜室，即细胞板，随后通过新到达的囊泡不断融合到其边缘而向外侧扩张[1-4]。囊泡的靶向递送是通过植物特异性细胞骨架阵列——膜质体的动态重组来辅助的，膜质体从一个固体圆柱体变成一个扩大的环状结构[6]。这种横向易位是由中心微管解聚，让位于膨胀的细胞板，以及微管沿边缘聚合引起的[6,7]。虽然已知有几种成分可介导细胞动力学囊泡融合[8-10]，但尚未发现通过突变介导细胞膜动力学的基因功能。拟南芥HINKEL基因的突变导致细胞分裂缺陷，如细胞壁不完整的细胞增大和多核。细胞动力学特异性合成素KNOLLE[8]的靶向性和膜质体的横向扩张不受影响。然而，膜质体微管似乎持续存在于中心，在那里囊泡融合应该导致细胞板形成。分子分析表明，HINKEL基因编码一种具有n端马达结构域的植物特异性激酶相关蛋白，并以类似于KNOLLE基因的细胞周期依赖方式表达。我们的研究结果表明，HINKEL在细胞膜微管的重组中发挥了作用。
Plant cytokinesis starts in the center of the division plane, with vesicle fusion generating a new membrane compartment, the cell plate, that subsequently expands laterally by continuous fusion of newly arriving vesicles to its margin [1-4]. Targeted delivery of vesicles is assisted by the dynamic reorganization of a plant-specific cytoskeletal array, the phragmoplast, from a solid cylinder into an expanding ring-shaped structure [6]. This lateral translocation is brought about by depolymerization of microtubules in the center, giving way to the expanding cell plate, and polymerization of microtubules along the edge [6, 7]. Whereas several components are known to mediate cytokinetic vesicle fusion [8-10], no gene function involved in phragmoplast dynamics has been identified by mutation. Mutations in the Arabidopsis HINKEL gene cause cytokinesis defects, such as enlarged cells with incomplete cell walls and multiple nuclei. Proper targeting of the cytokinesis-specific syntaxin KNOLLE [8] and lateral expansion of the phragmoplast are not affected. However, the phragmoplast microtubules appear to persist in the center, where vesicle fusion should result in cell plate formation. Molecular analysis reveals that the HINKEL gene encodes a plant-specific kinesin-related protein with a putative N-terminal motor domain and is expressed in a cell cycle-dependent manner similar to the KNOLLE gene. Our results suggest that HINKEL plays a role in the reorganization of phragmoplast microtubules during cell plate formation.