Effects of ammonium hexafluorosilicate application on demineralized enamel and dentin of primary teeth
Effects of ammonium hexafluorosilicate application on demineralized enamel and dentin of primary teeth
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DOI：
10.2334/josnusd.54.267
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发表时间：
2012-09-01
影响因子：
1.9
通讯作者：
Tay, Franklin R.
中科院分区：
文献类型：
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作者：
Hosoya, Yumiko;Watanabe, Eiko;Tay, Franklin R.
银二胺氟化物(Ag(NH3)(2)F)可以阻止龋齿，但会使牙齿染黑。为了克服这一缺点，我们应用了六氟硅酸铵(AHF；(NH4)(2)SiF6)，观察了脱矿乳牙脱矿釉质和牙本质的颜色和结构的变化。将牙釉质和牙本质在10%EDTA溶液中脱矿90 S，然后用35%磷酸凝胶脱矿60 S，然后浸泡在AHF溶液中60 S，在扫描电子显微镜和能谱分析之前，将牙釉质和牙本质在10%EDTA溶液中脱矿90 S。根据AHF应用和人工唾液浸泡情况将牙齿分为4组进行检查。采用单因素方差分析和Fisher‘s PLSD检验对数据进行统计学处理，P<；0.05。AHF处理未引起明显变色。AHF处理脱矿后出现的釉质棱柱和牙小管上覆盖有沉淀物。唾液浸泡组可见球状的膜性结构。EDS分析表明，AHF应用对牙釉质没有影响，但AHF应用后牙本质表面的F%和Ca/P值显著高于未浸泡人工唾液的牙本质表面。在防腐治疗方面还需要进一步研究。
Silver diamine fluoride (Ag(NH3)(2)F) arrests caries but stains teeth black. To overcome this drawback, we applied ammonium hexafluorosilicate (AHF; (NH4)(2)SiF6) and observed changes in the color and structure of demineralized enamel and dentin of extracted primary teeth. Enamel and dentin were demineralized in 10% EDTA solution for 90 s followed by 35% phosphoric acid gel for 60 s, then soaked in AHF solution for 60 s. Before analysis by scanning electron microscopy (SEM) and energydispersive X-ray spectrometry (EDS), enamel and dentin were demineralized in 10% EDTA for 90 s. Teeth were divided into 4 groups according to AHF application and artificial saliva immersion status and then examined. The data were statistically analyzed using one-way ANOVA and Fisher's PLSD test at a significance level of P < 0.05. AHF treatment did not cause visible discoloration. Enamel prisms and dental tubules appeared by demineralization were covered with precipitates by AHF application. A sphere-filled membranous structure was observed in the saliva immersion groups. EDS analysis showed that AHF application had no effect on enamel; however, F% and Ca/P ratio were significantly higher on dentin surfaces after AHF application without artificial saliva immersion. Further study on arresting caries treatment is required.