Patterns of extracellular norepinephrine in the paraventricular hypothalamus: relationship to circadian rhythm and deprivation-induced eating behavior.
Patterns of extracellular norepinephrine in the paraventricular hypothalamus: relationship to circadian rhythm and deprivation-induced eating behavior.
复制标题
室旁下丘脑细胞外去甲肾上腺素的模式：与昼夜节律和剥夺引起的饮食行为的关系。
DOI：
10.1016/0024-3205(89)90136-7
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发表时间：
1989
期刊：
影响因子：
6.1
通讯作者：
Leibowitz,SF
中科院分区：
文献类型：
--
作者：
Stanley,BG;Schwartz,DH;Hernandez,L;Hoebel,BG;Leibowitz,SF
为探讨下丘脑室旁核（PVN）内去甲肾上腺素（NE）的生理作用，采用微透析和高压液相色谱电化学检测法，测定了11只自由活动大鼠PVN细胞外内源性NE水平的变化。为了确定在自由进食的受试者中是否存在细胞外NE的昼夜节律模式，收集来自PVN附近的透析液样品并每2小时测定NE，持续48小时。在两个24小时期间，受试者之间的NE平均模式相似，在黑暗周期开始时有一个可靠的峰值，在所有其他时间都有相对稳定的水平。当这些动物随后被剥夺食物24小时，细胞外NE逐渐上升，观察到，最终增加到215%的predeprivation水平。当动物被重新喂食并以更频繁的间隔继续NE测量时，发现细胞外水平在进食的前20分钟内以及在接下来的3小时内随着食物摄入量减少而下降。这些模式的细胞外NE，加上以前的证据表明，内源性NE在PVN中起着启动和/或维持正常的饮食行为在开始的夜间喂养期，以及食物剥夺后的作用。
In order to clarify the physiological role of norepinephrine (NE) in the hypothalamic paraventricular nucleus (PVN), changes in extracellular levels of endogenous NE were measured in 11 freely-moving rats using microdialysis and high pressure liquid chromatography with electrochemical detection. To determine whether there was a circadian pattern of extracellular NE in freely-eating subjects, samples of dialysate from the vicinity of the PVN were collected and assayed for NE every 2 hrs for 48 hrs. The pattern of NE averaged across subjects was similar during both 24-hr periods, with a reliable peak at the beginning of the dark cycle and relatively stable levels at all other times. When these animals were subsequently deprived of food for 24 hrs, a gradual rise in extracellular NE was observed, ultimately increasing to 215% of the predeprivation level. When the animals were refed and NE measurements were continued at more frequent intervals, extracellular levels were found to decline during the first 20 min of eating, as well as over the next 3 hrs as food intake diminished. These patterns of extracellular NE, together with previous evidence, suggest that endogeneous NE in the PVN plays a role in the initiation and/or maintenance of normal eating behavior at the beginning of the nocturnal feeding period, as well as after food deprivation.