Electrochemiluminescence detection based on Ruthenium(II) tris(bipyridine) immobilised in sulfonic-functionalised titania nanoparticles by ion exchange strategy
Electrochemiluminescence detection based on Ruthenium(II) tris(bipyridine) immobilised in sulfonic-functionalised titania nanoparticles by ion exchange strategy
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DOI：
10.1039/b903434j
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发表时间：
2009-01-01
期刊：
影响因子：
4.2
通讯作者：
Yang, Xiurong
中科院分区：
文献类型：
--
作者：
Li, Yali;Yang, Fan;Yang, Xiurong
研制了一种新型钌（II）-三联吡啶固体电化学发光（ECL）传感器。通过离子交换法将三（2，2 '-联吡啶）钌（Ru（bpy）（3）（2+））固定在磺酸基多孔二氧化钛（TiO 2-SO 3 H）纳米粒子中，然后利用环境友好且稳定的生物高分子壳聚糖（CHIT）将Ru（bpy）（3）（2+）/TiO 2-SO 3 H包埋在ITO电极上，制备了该传感器。该传感器具有良好的电化学发光性能，线性范围为7.5 × 10 ~（-9）~ 2.5 × 10 ~（-3）M（R = 0.98），检出限为1.67 × 10 ~（-10）M（S/N = 3）。此外，该方法具有良好的重现性，连续电位扫描34个循环，ECL强度-时间曲线的相对平均偏差为0.95%。对于DBAE的检测也具有良好的长期稳定性。在使用三周后，该传感器能够对2.5 × 10（-4）M的DBAE保持超过80%的活性。该方法制备的电化学发光传感器简单易行，在电化学发光分析检测中具有潜在的应用价值。
A novel Ruthenium(II) tris(bipyridine)-based solid-state electrochemiluminescence (ECL) sensor was developed in this paper. The sensor was fabricated by immobilising tris(2,2'-bipyridyl) ruthenium(II) (Ru(bpy)(3)(2+)) in sulfonic-functionalised porous titania (TiO2-SO3H) nanoparticles via an ion exchange strategy, followed by employing environment friendly and stable biopolymer chitosan (CHIT) to entrap Ru(bpy)(3)(2+)/TiO2-SO3H onto the ITO electrode. The prepared ECL sensor exhibited excellent ECL behaviors and a wide linear range from 7.5 x 10(-9) to 2.5 x 10(-3) M (R = 0.98) with a detection limit of 1.67 x 10(-10) M (S/N = 3) towards 2-(dibutylamino)-ethanol (DBAE) detection. In addition, it had good reproducibility, and the relative average deviation was 0.95% of ECL intensity-time curve under continuous potential scanning for 34 cycles. It also had good long-term stability for the DBAE detection. After being used in three weeks, the sensor was able to keep over 80% activity towards 2.5 x 10(-4) M of DBAE. Fabrication of the ECL sensor by this method is simple and easy, and hence has potential applications in ECL analysis and detection.