α1-Tubulin FaTuA1 plays crucial roles in vegetative growth and conidiation in Fusarium asiaticum
α1-Tubulin FaTuA1 plays crucial roles in vegetative growth and conidiation in Fusarium asiaticum
复制标题
DOI：
10.1016/j.resmic.2015.01.001
复制
发表时间：
2015-04-01
影响因子：
2.6
通讯作者：
Ma, Zhonghua
中科院分区：
文献类型：
--
作者：
Hu, Weiqun;Zhang, Xiaoping;Ma, Zhonghua
丝状子囊菌Fusarium asiaticum含有两个编码α-微管蛋白的同源基因FaTUA 1和FaTUA 2。在本研究中，我们发现FaTUA 2在F.亚洲人。然而，FaTUA 1的缺失导致菌丝生长显著减少，菌丝扭曲，菌丝顶端细胞核异常。FaTuA 1缺失突变体（Delta FaTuA 1 -5）也显示出分生孢子的显著减少，并且产生异常分生孢子。致病性测定表明，Delta FaTuA 1 -5对小麦穗部的致病力降低。出乎意料的是，FaTUA 1的缺失导致了对高温的抗性。此外，Delta FaTuA 2对多菌灵的敏感性增加。此外，在Delta FaTuA 1 -5中，FaTUA 2表达量的增加部分恢复了突变体在菌丝生长、产孢和毒力方面的缺陷，而在FaTUA 2缺失突变体中，FaTUA 1表达量的增加也部分缓解了突变体在孢子萌发延迟方面的缺陷。这些结果表明，FaTuA 1和FaTuA 2在营养生长和发育中起着重要作用，FaTuA 1和FaTuA 2的功能在F.亚洲人。(C)2015巴斯德研究所。由Elsevier Masson SAS出版。All rights reserved.
The filamentous ascomycete Fusarium asiaticum contains two homologous genes FaTUA1 and FaTUA2 encoding alpha-tubulins. In this study, we found that FaTUA2 was dispensable for vegetative growth and sporulation in F. asiaticum. The deletion of FaTUA1 however led to dramatically reduced mycelial growth, twisted hyphae and abnormal nuclei in apical cells of hyphae. The FaTUA1 deletion mutant (Delta FaTuA1-5) also showed a significant decrease in conidiation, and produced abnormal conidia. Pathogenicity assays showed that Delta FaTuA1-5 exhibited decreased virulence on wheat head. Unexpectedly, the deletion of FaTUA1 led to resistance to high temperatures. In addition, Delta FaTuA2 showed increased sensitivity to carbendazim. Furthermore, increased FaTUA2 expression in Delta FaTuA1-5 partially restored the defects of the mutant in mycelial growth, conidial production and virulence, vice versa, increased FaTUA1 expression in the FaTUA2 deletion mutant also partially relieved the defect of the mutant in the delay of conidial germination. Taken together, these results indicate that FaTuA1 plays crucial roles in vegetative growth and development, and the functions of FaTuA1 and FaTuA2 are partially interchangeable in F. asiaticum. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.