Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules
Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules
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DOI：
10.4161/psb.3.12.6848
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Ellis, Brian E.
中科院分区：
文献类型：
--
作者：
Lee, Jin Suk;Huh, Kyung Won;Ellis, Brian E.
拟南芥基因组编码一个由有丝分裂原激活蛋白激酶（MPKs）组成的20个成员的基因家族，但这些关键信号成分的生物学作用仅被确定为一小部分。特别是，目前还不清楚mpk是如何在细胞内组织成功能模块的。为了深入了解它们之间的潜在关系，我们使用酵母双杂交系统对所有拟南芥mpk及其上游激活因子（MAPK激酶；MKK）进行了定向蛋白-蛋白相互作用筛选。利用重组蛋白，还在体外测试了酶-底物功能的新型相互作用。结果数据证实了许多早期报道的MKK-MPK关系，但也揭示了更广泛的相互作用模式，这将有助于指导未来植物中MAPK信号的分析。
The Arabidopsis genome encodes a 20-member gene family of mitogen-activated protein kinases (MPKs) but biological roles have only been identified for a small subset of these crucial signalling components. In particular, it is unclear how the MPKs may be organized into functional modules within the cell. To gain insight into their potential relationships, we used the yeast two-hybrid system to conduct a directed protein-protein interaction screen between all the Arabidopsis MPKs and their upstream activators (MAPK kinases; MKK). Novel interactions were also tested in vitro for enzyme-substrate functionality, using recombinant proteins. The resulting data confirm a number of earlier reported MKK-MPK relationships, but also reveal a more extensive pattern of interactions that should help to guide future analyses of MAPK signalling in plants.