Quaranfil, Johnston Atoll, and Lake Chad Viruses Are Novel Members of the Family Orthomyxoviridae
Quaranfil, Johnston Atoll, and Lake Chad Viruses Are Novel Members of the Family Orthomyxoviridae
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DOI：
10.1128/jvi.00677-09
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发表时间：
2009-11-15
影响因子：
5.4
通讯作者：
Wang, David
中科院分区：
文献类型：
--
作者：
Presti, Rachel M.;Zhao, Guoyan;Wang, David
虫媒病毒感染是由于人类、动物和吸血节肢动物的运动而引起的新出现的感染的重要原因。Quaranfil病毒（QRFV）是一种未分类的虫媒病毒，最初于1953年在埃及Quaranfil从患有轻度发热疾病的儿童中分离出来。随后在多个地理区域从蜱和鸟类中分离出。我们使用高通量测序将QRFV归类为一种新的正粘病毒。该病毒的基因组由多个RNA片段组成;其中五个片段已被完全测序。与已知正粘病毒的聚合酶（PA，PB 1和PB 2）和血凝素（HA）基因中的保守结构域具有有限氨基酸相似性的蛋白质被预测存在于四个片段中。第五个测序片段与任何蛋白质都没有可检测到的相似性，并且功能不确定。QRFV的末端-末端序列在片段之间是保守的，并且与已知的正粘病毒属的那些不同。已知QRFV与其他两种未分类的病毒，约翰斯顿环礁病毒（JAV）和乍得湖病毒（LKCV）发生血清学交叉反应。对JAV的PB 1和HA的完整开放阅读框进行测序，而对LKCV的PB 1的片段进行质谱测序。QRFV和JAV PB 1和HA彼此分别共享80%和70%的氨基酸同一性; LKCV PB 1片段与QRFV PB 1的相应区域共享83%的氨基酸同一性。基于系统发育分析，病毒粒子的超微结构特征，和独特的末端-末端序列的确定，我们建议，QRFV，JAV，和LKCV包括一个新的属的家庭正粘病毒科。
Arboviral infections are an important cause of emerging infections due to the movements of humans, animals, and hematophagous arthropods. Quaranfil virus (QRFV) is an unclassified arbovirus originally isolated from children with mild febrile illness in Quaranfil, Egypt, in 1953. It has subsequently been isolated in multiple geographic areas from ticks and birds. We used high-throughput sequencing to classify QRFV as a novel orthomyxovirus. The genome of this virus is comprised of multiple RNA segments; five were completely sequenced. Proteins with limited amino acid similarity to conserved domains in polymerase (PA, PB1, and PB2) and hemagglutinin (HA) genes from known orthomyxoviruses were predicted to be present in four of the segments. The fifth sequenced segment shared no detectable similarity to any protein and is of uncertain function. The end-terminal sequences of QRFV are conserved between segments and are different from those of the known orthomyxovirus genera. QRFV is known to cross-react serologically with two other unclassified viruses, Johnston Atoll virus (JAV) and Lake Chad virus (LKCV). The complete open reading frames of PB1 and HA were sequenced for JAV, while a fragment of PB1 of LKCV was identified by mass sequencing. QRFV and JAV PB1 and HA shared 80% and 70% amino acid identity to each other, respectively; the LKCV PB1 fragment shared 83% amino acid identity with the corresponding region of QRFV PB1. Based on phylogenetic analyses, virion ultrastructural features, and the unique end-terminal sequences identified, we propose that QRFV, JAV, and LKCV comprise a novel genus of the family Orthomyxoviridae.