Cytotoxicity study of polyethylene glycol derivatives
Cytotoxicity study of polyethylene glycol derivatives
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聚乙二醇衍生物的细胞毒性研究
DOI：
10.1039/c7ra00861a
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发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Tao, Lei
中科院分区：
文献类型：
--
作者：
Liu, Guoqiang;Li, Yongsan;Tao, Lei
聚乙二醇（PEG）衍生物已广泛应用于生物相关研究。然而，PEG低聚物（具有不同的分子量）或基于PEG的单体（具有不同的链端基）实际上具有不同的化学和物理性质，这可能导致潜在的毒性。在这项工作中，使用人宫颈癌细胞 (HeLa) 和小鼠成纤维细胞系 (L929) 作为模型细胞，测量了一系列 PEG 衍生物（低聚物和单体）的细胞毒性。大多数 PEG 寡聚物对两种类型的细胞都是安全的，但三甘醇 (TEG) 除外，三甘醇 (TEG) 在高浓度下对 L929 细胞有毒。另一方面，聚乙二醇甲醚丙烯酸酯（mPEGA）和聚乙二醇甲醚甲基丙烯酸酯（mPEGMA）等基于PEG的单体表现出明显的细胞毒性。随后，人们对这些有毒的PEG衍生物进行了研究，揭示了它们不同的毒性机制。本研究评估了PEG衍生物的细胞毒性，并指出了“安全”生物材料的潜在危害，为人们在未来的生物医学研究中使用PEG衍生物提供了有益的参考。
Polyethylene glycol (PEG) derivatives have been widely used in bio-related research. However, PEG oligomers (with different molecular weights) or PEG based monomers (with different chain end groups) actually have different chemical and physical properties, which might lead to potential toxicity. In this work, the cytotoxicity of a series of PEG derivatives (oligomers and monomers) has been measured using human cervical cancer cells (HeLa) and a cell line of fibroblasts derived from mice (L929) as model cells. Most of the PEG oligomers are safe to both types of cells except triethylene glycol (TEG), which is toxic at high concentrations to L929 cells. On the other hand, PEG-based monomers including poly(ethylene glycol) methyl ether acrylate (mPEGA) and poly(ethylene glycol) methyl ether methacrylate (mPEGMA) showed obvious cytotoxicity. Subsequently, those toxic PEG derivatives have been studied to reveal the different mechanisms of their toxicity. This current research evaluated the cytotoxicity of PEG derivatives and pointed out the potential hazard of ‘safe’ biomaterials, which might offer a useful reference for people to use the PEG derivatives in future biomedical research.