Detection of Human Papillomavirus in Formalin‐Fixed, Invasive Squamous Carcinomas Using the Polymerase Chain Reaction
Detection of Human Papillomavirus in Formalin‐Fixed, Invasive Squamous Carcinomas Using the Polymerase Chain Reaction
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使用聚合酶链反应检测福尔马林固定的侵袭性鳞状癌中的人乳头瘤病毒
DOI：
10.1097/00000478-198903000-00007
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
P. Fitzgibbons
中科院分区：
文献类型：
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作者：
M. Kiyabu;D. Shibata;N. Arnheim;W. Martin;P. Fitzgibbons
我们分析了88例福尔马林固定、石蜡包埋的侵袭性鳞状癌患者的人乳头瘤病毒相关DNA序列（HPV 16型和18型），并使用聚合酶链反应进行体外基因扩增。50例（70%）肛门生殖区癌中有35例（70%）发现了HPV DNA序列，包括4例（100%）肛门癌、8例（75%）外阴癌、14例（64%）阴道癌、5例（40%）阴茎癌和19例（74%）宫颈肿瘤。25例口咽鳞状癌中有9例（36%）含有HPV DNA序列，包括10例喉癌中有4例（40%），8例颊癌中有3例（38%），7例舌癌中有2例（29%）。13例食管癌未检出HPV DNA序列。在44例含有病毒DNA的病例中，HPV-16检出41例（93%），HPV-18检出5例（11%），两种类型均在2例（肛门和外阴各1例）中检出。83例非疣状癌中有43例（52%）发现了HPV DNA序列，5例疣状癌中有1例（20%）发现了HPV DNA序列，但差异不显著。这些发现表明，HPV DNA序列与肛门生殖器比口咽鳞状癌更频繁地相关，并且可以使用聚合酶链反应在常规处理的组织中很容易检测到。
We analyzed 88 formalin-fixed, paraffin-embedded invasive squamous carcinomas for human papillomavirus-related DNA sequences (HPV types 16 and 18) following in vitro gene amplification using the polymerase chain reaction. HPV DNA sequences were found in 35 of 50 (70%) carcinomas of the anogenital region, including four of four (100%) anal, six of eight (75%) vulvar, nine of 14 (64%) vaginal, two of five (40%) penile, and 14 of 19 (74%) cervical tumors. Nine of 25 (36%) oropharyngeal squamous carcinomas contained HPV DNA sequences, including four of 10 (40%) laryngeal, three of eight (38%) buccal, and two of seven (29%) glossal tumors. HPV DNA sequences were not found in 13 esophageal carcinomas. Of the 44 cases that contained viral DNA, HPV-16 was detected in 41 cases (93%) and HPV-18 in five cases (11%), while both types were found in two cases (one anal and one vulvar). HPV DNA sequences were found in 43 of 83 (52%) nonverrucous and in one of five (20%) verrucous carcinomas, but this difference was not significant. These findings demonstrate that HPV DNA sequences are more frequently associated with anogenital than oropharyngeal squamous carcinomas and can be readily detected in routinely processed tissues using the polymerase chain reaction.