Synergistic interaction of Rhizobium leguminosarum bv. viciae and arbuscular mycorrhizal fungi as a plant growth promoting biofertilizers for faba bean (Vicia faba L.) in alkaline soil
Synergistic interaction of Rhizobium leguminosarum bv. viciae and arbuscular mycorrhizal fungi as a plant growth promoting biofertilizers for faba bean (Vicia faba L.) in alkaline soil
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DOI：
10.1016/j.micres.2013.07.007
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发表时间：
2014-01-01
影响因子：
6.7
通讯作者：
Morsy, Fatthy Mohamed
中科院分区：
文献类型：
--
作者：
Abd-Alla, Mohamed Hemida;El-Enany, Abdel-Wahab Elsadek;Morsy, Fatthy Mohamed
埃及土壤的 pH 值通常呈弱碱性至碱性（7.5-8.7），这主要是由于其干燥的环境造成的。在干旱和半干旱地区，盐浓度较低，钠以碳酸盐和碳酸氢盐形式占主导地位，这促进了碱性土壤的形成。碱性土壤由于物理性质差而存在肥力问题，对农作物的生长和产量产生不利影响。因此，本研究致力于研究根瘤菌和丛枝菌根真菌的协同相互作用，以改善碱性土壤中蚕豆的生长。共分离出20株根瘤菌和4种丛枝菌根真菌（AMF）。研究了根瘤菌分离株在碱性胁迫下的生长能力。从 20 个分离株中选择 3 个分离株作为耐受分离株。这 3 个根瘤菌分离株根据编码 16S rRNA 的基因序列进行鉴定，并命名为根瘤菌 sp。埃及 16 (HM622137)，根瘤菌 sp。埃及 27 (HM622138) 和豆根瘤菌。豌豆 STDF-埃及 19 (HM587713)。耐碱性最好的是豆科植物 R. leguminosarum。豌豆 STDF-埃及 19 (HM587713)。 R. leguminosarum 的作用。分别评估了蚕豆 STDF-Egypt 19 和 AMF（无茎孢子菌、地孢子菌、苔藓球囊菌和杏小孢子菌）混合物的单独和组合对碱性胁迫下蚕豆结瘤、固氮和生长的影响。与单独接种的植物相比，在双重接种的植物中记录到根瘤的数量和质量、固氮酶活性、根瘤的豆血红蛋白含量、菌根定殖、根和芽的干质量比对照显着增加。蚕豆固氮作用的增强可归因于 AMF 促进了某些元素的动员，如磷、铁、钾和其他参与固氮酶和豆血红蛋白合成的矿物质。由此可见，根瘤菌和AMF生物肥料双重接种对于促进碱性土壤中蚕豆的生长比单独接种更有效，反映出接种剂之间存在协同关系。 (C) 2013 爱思唯尔有限公司。版权所有。
Egyptian soils are generally characterized by slightly alkaline to alkaline pH values (7.5-8.7) which are mainly due to its dry environment. In arid and semi-arid regions, salts are less concentrated and sodium dominates in carbonate and bicarbonate forms, which enhance the formation of alkaline soils. Alkaline soils have fertility problems due to poor physical properties which adversely affect the growth and the yield of crops. Therefore, this study was devoted to investigating the synergistic interaction of Rhizobium and arbuscular mycorrhizal fungi for improving growth of faba bean grown in alkaline soil. A total of 20 rhizobial isolates and 4 species of arbuscular mycorrhizal fungi (AMF) were isolated. The rhizobial isolates were investigated for their ability to grow under alkaline stress. Out of 20 isolates 3 isolates were selected as tolerant isolates. These 3 rhizobial isolates were identified on the bases of the sequences of the gene encoding 16S rRNA and designated as Rhizobium sp. Egypt 16 (HM622137), Rhizobium sp. Egypt 27 (HM622138) and Rhizobium leguminosarum by. viciae STDF-Egypt 19 (HM587713). The best alkaline tolerant was R. leguminosarum by. viciae STDF-Egypt 19 (HM587713). The effect of R. leguminosarum by. viciae STDF-Egypt 19 and mixture of AMF (Acaulospora laevis, Glomus geosporum, Glomus mosseae and Scutellospora armeniaca) both individually and in combination on nodulation, nitrogen fixation and growth of Vicia faba under alkalinity stress were assessed. A significant increase over control in number and mass of nodules, nitrogenase activity, leghaemoglobin content of nodule, mycorrhizal colonization, dry mass of root and shoot was recorded in dual inoculated plants than plants with individual inoculation. The enhancement of nitrogen fixation of faba bean could be attributed to AMF facilitating the mobilization of certain elements such as P, Fe, K and other minerals that involve in synthesis of nitrogenase and leghaemoglobin. Thus it is clear that the dual inoculation with Rhizobium and AMF biofertilizer is more effective for promoting growth of faba bean grown in alkaline soils than the individual treatment, reflecting the existence of synergistic relationships among the inoculants. (C) 2013 Elsevier GmbH. All rights reserved.