Impact of queen infection on kitten susceptibility to different strains of Bartonella henselae
Impact of queen infection on kitten susceptibility to different strains of Bartonella henselae
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DOI：
10.1016/j.vetmic.2015.09.020
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发表时间：
2015-11-18
影响因子：
3.3
通讯作者：
Diniz, Pedro Paulo V. P.
中科院分区：
文献类型：
--
作者：
Fleischman, Drew A.;Chomel, Bruno B.;Diniz, Pedro Paulo V. P.
家猫是汉赛巴尔通体（Bartonella henselae）的天然宿主，人类猫抓病的病原体。在小猫中，母体IgG抗体在产后两周内可检测到，断奶在产后6至10周内，6至8周龄的小猫在自然环境中可能成为菌血症。该研究的目的是评估母体抗体是否针对特定的B。henselae菌株保护小猫免受来自相同基因型的相同菌株或不同菌株的感染。3只血清阴性、无巴尔通体的妊娠蜂王感染同一株B。henselae基因型II在怀孕期间。用与感染蚁后相同的菌株攻毒1号和2号蚁后的小猫，而用不同的基因型II菌株攻毒3号蚁后的小猫。攻毒后，来自感染相同菌株的母猫的所有小猫均发生血清转化，7只小猫中有6只未出现至极低水平的一过性菌血症。相反，用不同菌株攻毒的所有4只小猫均出现高菌血症（血液培养平均47，900 CFU/mL，定量PCR平均146，893个细菌/mL）。总体而言，所有小猫的qPCR和细菌培养物均具有良好的一致性（Kappa Cohen一致性为0.78）。本研究表明，幼猫很容易感染不同的B菌株。因此，即使在母源抗体存在的情况下，也可以在很小的年龄就发现henselae，这强调了在怀孕的女王和年轻的小猫中控制跳蚤的重要性。(C)2015 Elsevier B. V.版权所有。
Domestic cats are the natural reservoir of Bartonella henselae, the agent of cat scratch disease in humans. In kittens, maternal IgG antibodies are detectable within two weeks postpartum, weaning in six to ten weeks postpartum and kittens as young as six to eight weeks old can become bacteremic in a natural environment.The study's objective was to evaluate if maternal antibodies against a specific B. henselae strain protect kittens from infection with the same strain or a different strain from the same genotype. Three seronegative and Bartonella-free pregnant queens were infected with the same strain of B. henselae genotype II during pregnancy. Kittens from queens #1 and #2 were challenged with the same strain used to infect the queens while kittens from queen #3 were challenged with a different genotype II strain.All queens gave birth to non-bacteremic kittens. After challenge, all kittens from queens infected with the same strain seroconverted, with six out of the seven kittens presenting no to very low levels of transitory bacteremia. Conversely, all four kittens challenged with a different strain developed high bacteremia (average 47,900 CFU/mL by blood culture and 146,893 bacteria/mL by quantitative PCR). Overall, qPCR and bacterial culture were in good agreement for all kittens (Kappa Cohen's agreement of 0.78).This study demonstrated that young kittens can easily be infected with a different strain of B. henselae at a very young age, even in the presence of maternal antibodies, underlining the importance of flea control in pregnant queens and young kittens. (C) 2015 Elsevier B.V. All rights reserved.