Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft.
Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft.
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人胰腺癌相关抗原和癌胚抗原在同源胰腺肿瘤异种移植物中的差异定位。
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
T. Chu
中科院分区：
文献类型：
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作者：
M. H. Tan;T. Shimano;T. Chu
应用特异性抗PCAA和抗CEA抗血清的间接免疫荧光技术，研究了人胰腺癌相关抗原（PCAA）和癌胚抗原（CEA）在人胰腺癌腹水细胞系（AsPC-1）的组织定位。组织学上，AsPC-1异种移植物由不同大小和腺分化程度的粘液腺癌组成。PCAA选择性地与柱状细胞质结合，主要参与上皮细胞增殖，并起源于腺体的基底面。相反，CEA呈弥漫性，主要存在于立方细胞中，在管腔边缘和粘蛋白中具有较高的强度。此外，AsPC-1腺癌的生长比较显示，除了在形态学上与1级（G1）原位癌一致的幼年腺中，PCAA阳性染色细胞的数量从G1到4级上皮分化减少。在高分化腺癌和变性腺癌中，PCAA阳性细胞被释放到腺体的管腔中，而在低分化腺癌中，这些细胞通常分散在单个和/或灶性簇中，并且在极低分化腺癌中消失。结果表明，PCAA与CEA的免疫反应性及在同源肿瘤组织中的分布不同，PCAA主要与恶性上皮的增殖期有关。这些结果也表明了这种抗原表达在人类胰腺癌的病因学、疾病分级和分期中的潜在适用性。
Tissue localization of a human pancreas cancer-associated antigen (PCAA) and carcinoembryonic antigen (CEA) was studied in a homologous pancreatic tumoral xenograft, a human pancreatic cancer line established from ascites (AsPC-1), with the use of the indirect immunofluorescence technique with specific anti-PCAA and anti-CEA antisera. Histologically, AsPC-1 xenograft was composed of mucinous adenocarcinoma of variable size and degree of glandular differentiation. PCAA was selectively associated with the columnar cytoplasm, was involved primarily in the epithelial proliferation, and originated at the basal aspect of the glands. In contrast, CEA appeared diffuse and was found predominantly in the cuboidal cells, with higher intensity at the luminal border and in mucin. Furthermore, growth comparison of the AsPC-1 adenocarcinoma revealed that, except in the juvenile gland that was morphologically consistent with grade 1 (G1) carcinoma in situ, the number of PCAA-positively stained cells decreased from G1 to grade 4 epithelial differentiation. In well-differentiated adenocarcinoma and degenerating adenocarcinoma, cells positive for PCAA were released into the lumen of the glands, whereas in poorly differentiated adenocarcinoma these cells were often scattered singly and/or in focal clusters and disappeared in very poorly differentiated adenocarcinoma. This study showed that the PCAA was different from CEA in its immunologic reactivity and distribution in its homologous tumoral tissues, in which PCAA was predominantly associated with the proliferative phase of the malignant epithelium. These results also indicated the potential applicability of this antigenic expression in the etiology, disease grading, and staging of human pancreatic cancer.