Clinical evaluation of the serodiagnostic value of enzyme-linked immunosorbent assay for Rhodococcus equi infection in feats
Clinical evaluation of the serodiagnostic value of enzyme-linked immunosorbent assay for Rhodococcus equi infection in feats
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DOI：
10.1111/j.2042-3306.1997.tb03123.x
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发表时间：
1997-07-01
影响因子：
2.2
通讯作者：
Takai, S
中科院分区：
文献类型：
--
作者：
Higuchi, T;Hashikura, S;Takai, S
应用酶联免疫吸附测定法(ELISA)检测日本东高752匹表现出各种传染病征兆的患病马驹血清中抗ATCC 6939株吐温20提取抗原的抗体。在以往研究中，根据健康马血清的读数，将光密度(OD)值暂定为阳性。在两年的研究中，752匹患病马驹中有138匹的OD值在0.3或更高，被指定为“疑似马匹感染”。初检时年龄分布显著，多数(%)为31~60日龄，超过该日龄的受试者急剧减少。在138头疑似感染马立克原虫的马驹中，34匹(25%)初检时OD值超过0.9，除血白细胞计数、血清纤维蛋白原和α-球蛋白水平较高外，感染马驹在血清学诊断前和诊断后立即使用抗生素治疗，126匹(91%)马驹康复，但12匹(9%)临床症状没有改善。在尸检中，这些小马驹表现为化脓性肺炎和肠相关淋巴结炎并伴有腹部脓肿，所有来自肺和腹部脓肿的分离株均显示马立克氏杆菌。这些结果表明，OD读数在高范围内与马立克氏杆菌严重播散性感染有关。
An enzyme-linked immunosorbent assay (ELISA) for detection of serum IgG antibodies against Tween 20-extracted antigen of strain ATCC 6939 was applied in Hidaka, Japan to a total of 752 sick foals showing a variety of signs of infectious disease, An optical density (OD) value of more than 0.3 was tentatively fixed to be positive on the basis of readings made of healthy horse sera in previous studies, During a 2 year study, 138 of the 752 sick foals showed an OD value of 0.3 or higher and were designated as 'suspected of R. equi infection', Age distribution during the initial medical examination of the 138 seropositive foals was significant in that most (64%) foals were age 31-60 days, with a sharp decrease in subjects beyond that age, Of the 138 foals suspected of having R. equi infection, 34 foals (25%) showed OD values of over 0.9 at the initial medical examination, in addition to high blood leucocyte counts and serum fibrinogen and alpha-globulin values, The infectious foals had been treated with antibiotics just before and after serodiagnosis and 126 foals (91%) recovered from the disease, However, no clinical improvement was observed in 12 foals (9%). At necropsy, these foals revealed suppurative pneumonia and lymphadenitis of gut associated lymph nodes accompanied by abdominal abscesses, All isolates from the pulmonary and abdominal abscesses revealed R. equi. These results suggest that OD readings in the high range are associated with severe disseminated infection with R. equi.