Purification and immunochemical characterization of human plasma factor XIII.
Purification and immunochemical characterization of human plasma factor XIII.
复制标题
人血浆因子 XIII 的纯化和免疫化学表征。
DOI：
10.1159/000214153
复制
发表时间：
1976
期刊：
影响因子：
--
通讯作者：
R. Mcdonagh
中科院分区：
文献类型：
--
作者：
M. Kazama;J. Mcdonagh;R. Wagner;R. D. Langdell;R. Mcdonagh
对血浆因子XIII的纯化条件进行了详细的研究，并开发了最佳程序。该方法包括用氢氧化铝吸附，用硫酸铵反复分级分离，纤维蛋白原热变性，用聚乙二醇沉淀，冷沉淀，以及在DEAE-纤维素上进行梯度洗脱的色谱法。通过因子XIII的荧光胺掺入试验测量的终产物的比活性平均约为3，000 - 4，000 μ mol丹酰尸胺掺入/mg蛋白质。产率约为20%。在SDS电泳中，最终产物在非还原条件下有两条带，这是a和B亚基的特征。
A detailed study of the conditions for purification of plasma factor XIII was undertaken and an optimal procedure developed. This procedure involves adsorption with aluminum hydroxide, repeated fractionation with ammonium sulfate, heat denaturation of fibrinogen, precipitation with polyethylene glycol, cryoprecipitation, and chromatography on DEAE-cellulose with gradient elution. The specific activity of the final product, as measured by the fluorescent amine incorporation assay for factor XIII, averages about 3,000-4,000 mumol dansylcadaverine incorporation/mg protein. The yield is about 20%. In SDS electrophoresis the final product has two bands under nonreducing conditions, characteristic of the a and b subunits.