Single-molecule fluorescence-based studies on the dynamics, assembly and catalytic mechanism of the spliceosome.
Single-molecule fluorescence-based studies on the dynamics, assembly and catalytic mechanism of the spliceosome.
复制标题
基于单分子荧光的剪接体动力学、组装和催化机制的研究。
DOI：
10.1042/bst20140105
复制
发表时间：
2014
影响因子：
3.9
通讯作者：
Rueda,David
中科院分区：
文献类型：
--
作者：
Warnasooriya,Chandani;Rueda,David
Pre-mRNA (precursor mRNA) splicing is a key step in cellular gene expression where introns are excised and exons are ligated together to produce mature mRNA. This process is catalysed by the spliceosome, which consists of five snRNPs (small nuclear ribonucleoprotein particles) and numerous protein factors. Assembly of these snRNPs and associated proteins is a highly dynamic process, making it challenging to study the conformational rearrangements and spliceosome assembly kinetics in bulk studies. In the present review, we discuss recent studies utilizing techniques based on single-molecule detection that have helped overcome this challenge. These studies focus on the assembly dynamics and splicing kinetics in real-time, which help understanding of spliceosomal assembly and catalysis.
