RFLP-based genetic map of the homoeologous group 3 chromosomes of wheat and rye
RFLP-based genetic map of the homoeologous group 3 chromosomes of wheat and rye
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DOI：
10.1007/bf00232953
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发表时间：
1992-05-01
影响因子：
5.4
通讯作者：
Gale, M. D.
中科院分区：
文献类型：
--
作者：
Devos, K. M.;Atkinson, M. D.;Gale, M. D.
利用22个DNA探针和2个同工酶标记系统，构建了小麦3A、3B、3D染色体和黑麦3R染色体的遗传图谱。49个位点的分析映射显示极端的聚类在所有四个地图中的着丝粒周围，与大的“差距”在远端染色体区域，这被解释为是由于强本地化的重组对小麦和黑麦染色体的末端。在着丝粒区域，基因顺序在三个小麦基因组和黑麦基因组之间是高度保守的。然而，在染色体远端定位的DNA克隆的不可预测的行为可能表明，基因组在重组程度较高的区域中分化最快。cDNA和基因组探针的比较表明，后者是更有效地揭示RFLP。一些类别的gDNA克隆，即染色体特异性序列和以非同源方式杂交的那些，被认为是最多态的。图谱位置和RFLP水平之间的相关性没有明显的关系。除了匿名的DNA克隆，已知的功能克隆，景天庚酮糖-1，7-二磷酸酶（XSbp），羧肽酶I（XCxp 1）和bZIP蛋白（XEmbp）的位置，被确定沿着与两个同工酶位点，Mal-1和Est-5。
Genetic maps of chromosomes 3A, 3B and 3D of wheat and 3R of rye were developed using 22 DNA probes and two isozyme marker systems. Analysis of the 49 loci mapped showed extreme clustering around the centromere in all four maps, with large 'gaps' in the distal chromosome regions, which is interpreted as being due to strong localisation of recombination towards the ends of the wheat and rye chromosomes. In the centromeric regions gene orders are highly conserved between the three wheat genomes and the rye genome. However, the unpredictable behaviour of the DNA clones that map in distal chromosome locations may indicate that the genomes are diverging most rapidly in the regions of higher recombination. A comparison of cDNA and genomic probes showed the latter to be much more efficient for revealing RFLP. Some classes of gDNA clones, i.e. chromosome-specific sequences and those hybridizing in a non-homoeologous manner, were seen to be most polymorphic. Correlations between map locations and RFLP levels showed no clear relationship. In addition to anonymous DNA clones, the locations of known function clones, sedoheptulose-1,7-bisphosphatase (XSbp), carboxypeptidase I (XCxp1) and a bZIP protein (XEmbp), were ascertained along with those for two isozyme loci, Mal-1 and Est-5.