Electroimmunoassay, radioimmunoassay, and radial immunodiffusion assay evaluated for quantification of human apolipoprotein B.
Electroimmunoassay, radioimmunoassay, and radial immunodiffusion assay evaluated for quantification of human apolipoprotein B.
复制标题
评估电免疫测定、放射免疫测定和放射免疫扩散测定对人载脂蛋白 B 的定量。
作者：
M. D. Curry;A. Gustafson;P. Alaupovic;W. Mcconathy
我们研究了三种用于测量正常血脂和高脂蛋白血症患者血清载脂蛋白B和主要脂蛋白密度部分的免疫测定技术，并将电免疫测定法、放射免疫测定法和径向免疫扩散测定法与重量法测定的值进行了比较。电免疫分析法比放射免疫分析法更快、更简单，而且同样精确（两者的测定内和测定间变异系数分别为5%和7%）。所有免疫分析结果均与重量法测定正常血脂血清及其相应的脂蛋白密度部分的结果一致，但只有电免疫分析结果与重量法测定高甘油三酯患者分离的d < 1.019 g/ml的脂蛋白中载脂蛋白B的结果一致。电免疫法测定正常人和原发性高脂血症患者血浆载脂蛋白B浓度为：正常人980 +/- 200；I型，700 +/- 160；IIa型，2000 +/- 260；IIb型，2180 +/- 300；III型1300 +/- 340；IV型，1470 +/- 400；和V型，1550 +/- 390毫克/升（平均+/- SD）。来自高脂血症患者的脂蛋白密度分数均具有脂蛋白家族B的游离和相关形式的特征性分布。载脂蛋白B在游离和相关形式的脂蛋白B之间的绝对浓度和分布可能是潜在生化缺陷的有用指标。
We examined three immunoassay techniques for measuring apolipoprotein B in serum and major lipoprotein density fractions from normolipidemic and hyperlipoproteinemic persons, comparing values by electroimmunoassay, radioimmunoassay, and radial immunodiffusion assay with those determined gravimetrically. Electroimmunoassay is faster and simpler than radioimmunoassay, and equally precise (within- and between-assay coefficients of variation for both were 5 and 7%, respectively). All the immunoassays gave results that agreed with those by gravimetry for normolipidemic sera and the corresponding lipoprotein density fractions, but only electroimmunoassay results agreed with those by gravimetry for apolipoprotein B in lipoproteins of d less than 1.019 g/ml isolated from hypertriglyceridemic patients. Concentrations of apolipoprotein B in plasma, determined by electroimmunoassay in a population of normal persons and patients with primary hyperlipoproteinemias, were: normals, 980 +/- 200; type I, 700 +/- 160; type IIa, 2000 +/- 260; type IIb, 2180 +/- 300; type III, 1300 +/- 340; type IV, 1470 +/- 400; and type V, 1550 +/- 390 mg/liter (mean +/- SD). Lipoprotein density fractions from the hyperlipoproteinemic patients each had a characteristic distribution of free and associated forms of lipoprotein family B. The absolute concentration and distribution of apolipoprotein B between the free and associated forms of lipoprotein B may represent a useful indicator of the underlying biochemical defect(s).