Altered microRNA expression following sciatic nerve resection in dorsal root ganglia of rats
Altered microRNA expression following sciatic nerve resection in dorsal root ganglia of rats
复制标题
大鼠坐骨神经切除后背根神经节 microRNA 表达的改变
DOI：
10.1093/abbs/gmr083
复制
发表时间：
2011-11-01
影响因子：
3.7
通讯作者：
Gu, Xiaosong
中科院分区：
文献类型：
--
作者：
Yu, Bin;Zhou, Songlin;Gu, Xiaosong
MicroRNA (miRNA) 是一类小型非编码 RNA（类似于 22 个核苷酸），通过翻译抑制或目标 mRNA 的降解，在转录后负向调节基因表达。我们利用微阵列分析发现，大鼠坐骨神经切除后 1、4、7 和 14 天后，背根神经节 (DRG) 中 miRNA 的表达发生了以前未知的变化。在坐骨神经损伤后的四个时间点，DRG 中发现了 32 个显着上调和 18 个下调的 miRNA。通过实时 Taqman 聚合酶链式反应分析，四种连续失调的 miRNA 的表达与微阵列数据一致（上调：miR-21、miR-221；下调：miR-500、miR-551b）。这些 miRNA 的潜在靶标在坐骨神经切除后发生改变，主要涉及神经系统发育、多细胞有机体发育和细胞过程的调节。这项研究证明了在大鼠模型中切除坐骨神经后 miRNA 在 DRG 中的不同参与，并且它也可能有助于阐明负责神经再生的分子机制。
MicroRNAs (miRNAs) are a class of small, non-coding RNAs (similar to 22 nucleotides) that negatively regulate gene expression post-transcriptionally, either through translational inhibition or degradation of target mRNAs. We uncovered a previously unknown alteration in the expression of miRNAs in the dorsal root ganglia (DRG) at 1, 4, 7, and 14 days after resection of the sciatic nerve in rats using microarray analysis. Thirty-two significantly upregulated and 18 downregulated miRNAs were identified in the DRG at four time points following sciatic nerve injury. The expression of four consecutively deregulated miRNAs, analyzed by real-time Taqman polymerase chain reaction, was in agreement with the microarray data (upregulated: miR-21, miR-221; downregulated: miR-500, miR-551b). The potential targets for these miRNAs, altered after sciatic nerve resection, are involved mainly in nervous system development, multi-cellular organismal development, and the regulation of cellular processes. This study demonstrated a different involvement of miRNAs in the DRG after resection of the sciatic nerve in a rat model, and it may also contribute in illustrating the molecular mechanisms responsible for nerve regeneration.