AN AUTOREGULATORY ENHANCER ELEMENT OF THE DROSOPHILA HOMEOTIC GENE DEFORMED
AN AUTOREGULATORY ENHANCER ELEMENT OF THE DROSOPHILA HOMEOTIC GENE DEFORMED
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DOI：
10.1002/j.1460-2075.1990.tb07877.x
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发表时间：
1990-12-01
期刊：
影响因子：
11.4
通讯作者：
MCGINNIS, W
中科院分区：
文献类型：
--
作者：
BERGSON, C;MCGINNIS, W
果蝇胚胎前后不同区域的稳定决定受同源异型选择基因的持续表达控制。 有人提出一种机制来解释同源异型基因变形的持续表达，这是一种自激活回路，一旦变形表达被早期作用的模式基因建立起来，就会使用这种回路。 在这里，我们表明，一个大的顺式调控元件映射约5 kb的变形转录起始上游的变形autoregulatory增强预测的属性。 该元件在上颌骨和下颌骨段的表皮细胞中提供晚期的空间定位表达，其完全依赖于内源性畸形功能。此外，自身调节增强子可以通过畸形蛋白的异位表达在胚胎和成虫盘细胞中异位激活。 自身调节元件的缺失分析表明，它含有类似于其他同源异型位点的隔室特异性子元件。
The stable determination of different anterior - posterior regions of the Drosophila embryo is controlled by the persistent expression of homeotic selector genes. One mechanism that has been proposed to explain the persistent expression of the homeotic gene Deformed is an autoactivation circuit that would be used once Deformed expression had been established by earlier acting patterning genes. Here we show that a large cis-regulatory element mapping approximately 5 kb upstream of the Deformed transcription start has the properties predicted for a Deformed autoregulatory enhance. This element provides late, spatially localized expression in the epidermal cells of the maxillary and mandibular segments which is wholly dependent upon endogenous Deformed function. In addition, the autoregulatory enhancer can be activated ectopically in embryos and in imaginal disc cells by ectopic expression of Deformed protein. Deletion analysis of the autoregulatory element indicates that it contains compartment specific sub-elements similar to those of other homeotic loci.