Multiple amino acids in the glycoprotein of rabies virus are responsible for pathogenicity in adult mice
Multiple amino acids in the glycoprotein of rabies virus are responsible for pathogenicity in adult mice
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DOI：
10.1016/j.virusres.2005.08.004
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发表时间：
2006-02-01
期刊：
影响因子：
5
通讯作者：
Minamoto, N
中科院分区：
文献类型：
--
作者：
Takayama-Ito, M;Ito, N;Minamoto, N
我们已经报道了狂犬病西原毒株糖蛋白中164-303氨基酸之间的区域对成年小鼠的致死率是重要的。该区域包含西原强毒株和RC-HL强毒株之间的9个氨基酸替换。为了确定致病力的关键残基，产生了两个嵌合菌株和七个突变体，并对其进行了致病性检测。R(G 242/255/268)株基因组骨架第242、255和268位氨基酸被西原株取代，对小鼠的致死率与西原株相同。用西原菌株的氨基酸替换这三个氨基酸中的一个或两个的突变体不会恢复到R(G 242/255/268)菌株的致死性。这些结果表明，至少这三种氨基酸与致病性增强有关。目前认为G蛋白的多种氨基酸与狂犬病病毒的致病性有关。(C)2005 Elsevier B.V.保留所有权利。
We have reported that the region between amino acids 164 and 303 in the glycoprotein of rabies Nishigahara strain is important for lethality in adult mice. The region contains nine amino acid substitutions between the virulent Nishigahara and the avirulent RC-HL strains. In order to determine key residues for the pathogenicity, two chimeric strains and seven mutants were generated and examined for pathogenicities. The R(G 242/255/268) strain, in which amino acids at positions 242, 255, and 268 were replaced by those from the Nishigahara strain in the genomic backbone of the RC-HL strain, showed the same lethality as that of the Nishigahara strain in mice. Mutants in which one or two of these three amino acids were replaced by those from the Nishigahara strain did not revert to the lethality of the R(G 242/255/268) strain. These results demonstrate that at least these three amino acids are related to enhancement of pathogenicity. It is thought that multiple amino acids of the G protein are related to the pathogenicity of rabies viruses. (c) 2005 Elsevier B.V. All rights reserved.