Effect of buffer pH and peptide composition on the selectivity of peptide separations by capillary zone electrophoresis.
Effect of buffer pH and peptide composition on the selectivity of peptide separations by capillary zone electrophoresis.
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DOI：
10.1016/0003-2697(88)90188-1
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发表时间：
1988-09
影响因子：
2.9
通讯作者：
P. D. Grossman;K. Wilson;G. Petrie;H. Lauer
中科院分区：
文献类型：
--
作者：
P. D. Grossman;K. Wilson;G. Petrie;H. Lauer
一系列的10个合成肽含有不同程度的电荷和疏水性被用来研究肽的组成和缓冲液的pH值的毛细管区带电泳（CZE）的分离的选择性的影响。一个简单的模型被用来解释缓冲液的pH值对分离的影响。结果表明，pH值是影响CZE分离选择性的重要参数。此外，它表明，分离的选择性是这样的，不同的中性氨基酸组成的肽可以解决，甚至可以检测到肽的氨基酸序列的差异。β-乳球蛋白A的蛋白酶消化显示为复杂肽混合物分离的实际实例。
A series of 10 synthetic peptides containing varying degrees of charge and hydrophobicity was used to study the effects of peptide composition and buffer pH on the selectivity of separations by capillary zone electrophoresis (CZE). A simple model is used to explain the effect of buffer pH on the separation. It was found that pH is an important parameter affecting the selectivity of CZE separations. Furthermore, it is shown that the selectivity of the separation is such that peptides differing in neutral amino acid composition can be resolved, and that even differences in a peptide's amino acid sequence can be detected. A protease digest of β-lactoglobulin A is shown as a practical example of a separation of a complex peptide mixture.