The Ras-MAPK pathway is important for olfaction in Caenorhabditis elegans
The Ras-MAPK pathway is important for olfaction in Caenorhabditis elegans
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DOI：
10.1038/35005101
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发表时间：
2000-03-16
期刊：
影响因子：
64.8
通讯作者：
Ilno, Y
中科院分区：
文献类型：
--
作者：
Hirotsu, T;Saeki, S;Ilno, Y
众所周知，Ras-MAPK（丝裂原激活蛋白激酶）信号转导途径可响应细胞外信号控制细胞增殖和分化，但其其他功能尚不清楚。在秀丽隐杆线虫中，该通路调节多种发育事件，例如外阴诱导和减数分裂进展(1)，但其在神经系统中的功能尚不清楚。在这里，我们报告 Ras-MAPK 通路参与该生物体的嗅觉。该途径的突变失活和过度激活会损害一组气味剂的趋化效率。使用热休克启动子和细胞特异性启动子表达let-60 ras的实验表明，成熟嗅觉神经元需要LET-60 Ras的正常活性。将有气味的异戊醇应用于野生型动物会在 10 秒内激活嗅觉神经元中的 MAP 激酶。这种诱导取决于核苷酸门控通道 TAX-2/TAX-4 和电压激活钙通道亚基 UNC-2 的功能。这些结果表明 Ras-MAPK 通路在嗅觉神经元感觉信号的感知和传递中发挥动态调节作用。
The Ras-MAPK (mitogen-activated protein kinase) signal transduction pathway is well known to control cellular proliferation and differentiation in response to extracellular signals, but its other functions are less understood. In Caenorhabditis elegans this pathway regulates several developmental events, such as vulval induction and progression of meiosis(1), but its function in the nervous system is unknown. Here we report that the Ras-MAPK pathway is involved in olfaction in this organism. Mutational inactivation and hyperactivation of this pathway impairs efficiency of chemotaxis to a set of odorants. Experiments in which let-60 ras was expressed using a heat-shock promoter and a cell-specific promoter show that a normal activity of LET-60 Ras is required in mature olfactory neurons. Application of the odorant isoamylalcohol to wild-type animals leads to the activation of MAP kinase in olfactory neurons within 10 seconds. This induction is dependent on the function of the nucleotide-gated channel TAX-2/TAX-4 and the voltage-activated calcium channel subunit UNC-2. These results suggest a dynamic regulatory role for the Ras-MAPK pathway in perception and transmission of sensory signals in olfactory neurons.