Filaggrin, major basic protein and leukotriene B4: Biomarkers for adult patients of bronchial asthma, atopic dermatitis and allergic rhinitis
Filaggrin, major basic protein and leukotriene B4: Biomarkers for adult patients of bronchial asthma, atopic dermatitis and allergic rhinitis
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DOI：
10.5582/irdr.2018.01111
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发表时间：
2018-11-01
影响因子：
1.3
通讯作者：
Alzolibani, Abdullateef A.
中科院分区：
文献类型：
--
作者：
Bin Saif, Ghada A.;Rasheed, Zafar;Alzolibani, Abdullateef A.
支气管哮喘(BA)、特应性皮炎(AD)和变应性鼻炎(AR)是众所周知的具有复杂病因的特应性疾病。本研究旨在探讨微丝蛋白、嗜酸性粒细胞主要碱性蛋白(MBP)和白三烯B4(LTB4)在BA、AD和AR患者中的作用。收集1246例不同类型特应性疾病患者和410例正常对照组的血清，用双抗体夹心ELISA法检测血清微丝蛋白、MBP和LTB4，并以免疫球蛋白E(IgE)作为阳性对照。血清微丝蛋白水平在AD患者和多种(混合型)特应性疾病患者中显著升高(p<；0.001)，而在BA和AR患者中水平较低，但明显高于正常人血清(p<；0.01)。AR、BA和混合性异位症患者的MBP水平也较高，而AD患者的MBP水平无明显升高(p>；0.05)。相反，与正常人血清中存在的白蛋白4水平相比，所有受试的特应性患者组的白蛋白水平都显著降低(p<；0.001)。总而言之，这些发现支持微丝蛋白、MBP或LTB4与特应性疾病之间的联系。我们的数据有力地表明，微丝蛋白、MBP或LTB4可能有助于阐明这些特应性疾病的发病机制。
Bronchial asthma (BA), atopic dermatitis (AD), and allergic rhinitis (AR) are well known atopic disorders with complex etiologies. This study was undertaken to investigate the role of filaggrin, eosinophil major basic protein (MBP) and leukotriene B4 (LTB4) in patients with BA, AD, and AR. Sera from 1,246 patients with different atopic disorders and 410 normal healthy controls were collected and were evaluated for filaggrin, MBP and LTB4 by specific sandwich ELISAs, whereas immunoglobulin E (IgE) was used as a positive control for atopic patients. Serum analysis showed that filaggrin levels were remarkably high in patients with AD and in patients with multiple (mixed) atopic disorders (p < 0.001), whereas its levels in BA and AR patients were low but much higher than in normal human sera (p < 0.01). MBP levels were also high in AR, BA and mixed atopic patients, whereas AD patients showed no increase of MBP (p > 0.05). In contrast, LTB4 level was found to be significantly low in all tested atopic patients groups as compared to the levels of LTB4 present in normal human sera (p < 0.001). In conclusion, these findings support an association between filaggrin, MBP or LTB4 and atopic disorders. Our data strongly suggest that filaggrin, MBP or LTB4 might be useful in elucidating the mechanisms involved in the pathogenesis of these atopic disorders.