Synthesis of polyhydroxyalkanoates from glucose that contain medium-chain-length monomers via the reversed fatty acid β-oxidation cycle in Escherichia coli.
Synthesis of polyhydroxyalkanoates from glucose that contain medium-chain-length monomers via the reversed fatty acid β-oxidation cycle in Escherichia coli.
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DOI：
10.1016/j.ymben.2014.05.004
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发表时间：
2014-07
影响因子：
8.4
通讯作者：
Q. Zhuang;Qian Wang;Quanfeng Liang;Q. Qi
中科院分区：
文献类型：
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作者：
Q. Zhuang;Qian Wang;Quanfeng Liang;Q. Qi
含中链长单体的聚羟基脂肪酸酯（mcl-PHA）具有上级的物理机械性能，具有广泛的应用。从不相关的和可再生的来源合成这样的mcl-PHA的挑战是利用导致前体（R）-3-羟基酰基-CoA形成的有效代谢途径。在这里，通过设计反向脂肪酸β-氧化循环，我们能够在大肠杆菌中直接从葡萄糖合成mcl-PHA。在删除主要硫酯酶后，工程菌E.大肠杆菌产生6.62wt%的细胞干重mcl-PHA杂聚物。此外，当使用来自施氏假单胞菌1317的低底物特异性PHA合酶时，重组E.大肠杆菌合成了12.10wt%的细胞干重scl-mcl PHA共聚物，其中21.18mol%是3-羟基丁酸酯，78.82mol%是中链长度单体。脂肪酸β-氧化循环的逆转为大肠杆菌合成mcl-PHA提供了一条有效的代谢途径。可以进一步优化大肠杆菌。
Polyhydroxyalkanoates that contain the medium-chain-length monomers (mcl-PHAs) have a wide range of applications owing to their superior physical and mechanical properties. A challenge to synthesize such mcl-PHAs from unrelated and renewable sources is exploiting the efficient metabolic pathways that lead to the formation of precursor (R)-3-hydroxyacyl-CoA. Here, by engineering the reversed fatty acid β-oxidation cycle, we were able to synthesize mcl-PHAs inEscherichia colidirectly from glucose. After deletion of the major thioesterases, the engineeredE. coliproduced 6.62 wt% of cell dry weight mcl-PHA heteropolymers. Furthermore, when a low-substrate-specificity PHA synthase fromPseudomonas stutzeri1317 was employed, recombinantE. colisynthesized 12.10 wt% of cell dry weight scl–mcl PHA copolymers, of which 21.18 mol% was 3-hydroxybutyrate and 78.82 mol% was medium-chain-length monomers. The reversed fatty acid β-oxidation cycle offered an efficient metabolic pathway for mcl-PHA biosynthesis inE. coliand can be further optimized.