Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)
Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)
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DOI：
10.1099/vir.0.80546-0
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发表时间：
2005-03-01
影响因子：
3.8
通讯作者：
Liu, M
中科院分区：
文献类型：
--
作者：
Liu, SW;Chen, JF;Liu, M
冠状病毒样病毒分别命名为孔雀/中国/LKQ3/2003(PF/CH/LKQ3/03)和TEAL/中国/LDT3/2003(tl/CH/LDT3/03)。部分基因组序列分析表明，这些分离株具有典型的禽冠状病毒S-3-M-5-N基因序列。PF/CH/LKQ3/03株的S蛋白、膜蛋白和核衣壳蛋白基因与鸡传染性支气管炎冠状病毒H120疫苗株(马萨诸塞州血清型)和其他马萨诸塞州血清型分离株有99%的同源性。此外，将PF/CH/LKQ3/03接种于鸡体内(无特定病原体)，未见明显的疾病症状。TL/CH/LDT3/03有一个与中国传染性支气管炎病毒(IBV)分离株有95%同源性的刺突蛋白基因，但更广泛的测序表明该分离株可能发生了重组。当tl/CH/LDT3/03接种到鸡体内时，可导致鸡因肾炎死亡。综合以上信息，Pf/CH/LKQ3/03株可能是IBV疫苗株的回复株，而tl/CH/LDT3/03株是重组产生的肾病原区IBV株。在野外条件下，IBV在家养孔雀和青蛙体内的复制和非致病性使人们对这些宿主作为IBV携带者的作用以及它们可能必须将病毒传播到易感鸡群的可能性提出了疑问。
Coronavirus-like viruses, designated peafowl/China/LKQ3/2003 (pf/CH/LKQ3/03) and teal/China/LDT3/2003 (tl/CH/LDT3/03), were isolated from a peafowl and a teal during virological surveillance in Guandong province, China. Partial genomic sequence analysis showed that these isolates had the S-3-M-5-N gene order that is typical of avian coronaviruses. The spike, membrane and nucleocapsid protein genes of pf/CH/LKQ3/03 had > 99 % identity to those of the avian infectious bronchitis coronavirus H120 vaccine strain (Massachusetts serotype) and other Massachusetts serotype isolates. Furthermore, when pf/CH/LKQ3/03 was inoculated experimentally into chickens (specific-pathogen-free), no disease signs were apparent. tl/CH/LDT3/03 had a spike protein gene with 95 % identity to that of a Chinese infectious bronchitis virus (IBV) isolate, although more extensive sequencing revealed the possibility that this strain may have undergone recombination. When inoculated into chickens, tl/CH/LDT3/03 resulted in the death of birds from nephritis. Taken together, this information suggests that pf/CH/LKQ3/03 might be a revertant, attenuated vaccine IBV strain, whereas tl/CH/LDT3/03 is a nephropathogenic field IBV strain, generated through recombination. The replication and non-pathogenic nature of IBV in domestic peafowl and teal under field conditions raises questions as to the role of these hosts as carriers of IBV and the potential that they may have to transmit virus to susceptible chicken populations.