Correlation of Cell Adhesive Behaviors on Superhydrophobic, Superhydrophilic, and Micropatterned Superhydrophobic/Superhydrophilic Surfaces to Their Surface Chemistry
Correlation of Cell Adhesive Behaviors on Superhydrophobic, Superhydrophilic, and Micropatterned Superhydrophobic/Superhydrophilic Surfaces to Their Surface Chemistry
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DOI：
10.1021/la904447c
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发表时间：
2010-06-01
期刊：
影响因子：
3.9
通讯作者：
Takai, Osamu
中科院分区：
文献类型：
--
作者：
Ishizaki, Takahiro;Saito, Nagahiro;Takai, Osamu
采用等离子体化学气相沉积和真空紫外辐照技术成功制备了微图案化超疏水/超亲水表面。研究了超疏水、超亲水和超疏水/超亲水表面的物理化学性质。与超疏水表面相比，超亲水表面上的粗糙结构保持完整。将微图案化的超疏水/超亲水表面用作细胞培养的支架。在微图案化的表面上，细胞以高度选择性的方式附着到超亲水区域。形成对应于所述图案的细胞的圆形微阵列。在超亲水区域之间的图案距离为200 μ m的微图案化表面上，细胞粘附在超亲水区域上并部分延伸到相邻细胞。相反，当超亲水区域之间的图案距离大于400 μ m时，细胞不延伸到相邻细胞。研究了细胞在超疏水和超亲水表面上的粘附行为。细胞在超疏水和超亲水两种表面上均能粘附和增殖。然而，在超疏水表面上，需要持续接触以促进细胞分裂和增殖。另一方面，细胞在接种后立即容易在超亲水表面上粘附和增殖。细胞粘附行为的这些差异诱导超亲水区域上的位点选择性细胞粘附。此外，蛋白质吸附行为起着重要作用的细胞粘附在平坦的疏水和亲水表面上也进行了检查。蛋白质在亲水表面的吸附量远大于在疏水表面的吸附量。
A micropatterned superhydrophobic/superhydrophilic surface was successfully fabricated by plasma CVD and VUV irradiation. Physicochemical properties of the superhydrophobic, superhydrophilic, and superhydrophobic/superhydrophilic surfaces were investigated. The roughness structures on the superhydrophilic surface remained intact compared to those of the superhydrophobic surface. The micropatterned superhydrophobic/superhydrophilic surface was used as a scaffold of cell culture. On the micropatterned surface, the cells attached to the superhydrophilic regions in a highly selective manner. forming circular microarrays of the cells corresponding to the pattern. On the micropatterned surface with pattern distances of 200 mu m between superhydrophilic regions, the cells adhered on the superhydrophilic regions and partly extended to the neighboring cells. In contrast, when the pattern distances between the superhydrophilic regions were more than 400 mu m, the cells did not extend to the neighboring cells. Cell adhesion behaviors on superhydrophobic and superhydrophilic surfaces were also examined. The cells adhered and proliferated on both superhydrophobic and superhydrophilic surfaces. However, on the superhydrophobic surface, constant contact to facilitate cell division and proliferation was required. On the other hand, the cells easily adhered and proliferated on the superhydrophilic surface immediately alter seeding. These differences in cell adhesion behavior induced site-selective cell adhesion on the superhydrophilic regions. Furthermore, protein adsorption behavior that plays an important role in cell adhesion on flat hydrophobic and hydrophilic surface was also examined. The amounts of the protein adsorption on the flat hydrophilic surface were much greater than those on the flat hydrophobic surface.