INDUCERS OF MAMMALIAN-CELL DIFFERENTIATION STIMULATE DOME FORMATION IN A DIFFERENTIATED KIDNEY EPITHELIAL-CELL LINE (MDCK)
INDUCERS OF MAMMALIAN-CELL DIFFERENTIATION STIMULATE DOME FORMATION IN A DIFFERENTIATED KIDNEY EPITHELIAL-CELL LINE (MDCK)
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DOI：
10.1073/pnas.76.3.1323
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
LEVER, JE
中科院分区：
文献类型：
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作者：
LEVER, JE
分化的狗肾上皮细胞系MDCK的细胞培养物自发形成由许多细胞组成的充满液体的圆顶或半囊，作为专门的上皮转运现象的表现。向MDCK细胞中添加已知为细胞培养物中哺乳动物细胞分化的有效诱导剂的广谱化合物引起圆顶形成频率的显著增加。极性化合物，如N，N-二甲基甲酰胺，二甲亚砜或N，N-二乙酰胺刺激增加圆顶形成后15-30小时。这些化合物诱导的圆顶被阻止蛋白质合成抑制剂或哇巴因，细胞松弛素B或长春碱。DNA合成的抑制并没有阻止圆顶的化学诱导。其他诱导剂是生理发生的化合物，如正丁酸或腺苷。各种条件下，预计提高细胞内水平的环[c]AMP也刺激圆顶形成。在细胞培养物中，可以通过cAMP阳性控制下的细胞分化形式形成圆顶。
Cell cultures of a differentiated dog kidney epithelial cell line, MDCK, spontaneously form fluid-filled domes or hemicysts composed of numbers of cells as a manifestation of specialized epithelial transport phenomena. Addition to MDCK cells of a broad spectrum of compounds that are known as potent inducers of mammalian cell differentiation in cell culture caused a striking increase in the frequency of dome formation. Polar compounds such as N,N-dimethylformamide, dimethyl sulfoxide or hexamethylene bisacetamide stimulated increased dome formation 15-30 h after addition. Induction of domes by these compounds was prevented either by inhibitors of protein synthesis or by ouabain, cytochalasin B or vinblastine. Inhibition of DNA synthesis did not block chemical induction of domes. Other inducers were compounds of physiological occurrence such as n-butyrate or adenosine. A variety of conditions expected to elevate intracellular levels of cyclic[c]AMP also stimulated dome formation. Domes may be formed in cell culture by a form of cell differentiation under positive control by cAMP.