Transcriptional response of Medicago truncatula sulphate transporters to arbuscular mycorrhizal symbiosis with and without sulphur stress
Transcriptional response of Medicago truncatula sulphate transporters to arbuscular mycorrhizal symbiosis with and without sulphur stress
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DOI：
10.1007/s00425-012-1645-7
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发表时间：
2012-06-01
期刊：
影响因子：
4.3
通讯作者：
Wipf, Daniel
中科院分区：
文献类型：
--
作者：
Casieri, Leonardo;Gallardo, Karine;Wipf, Daniel
硫是植物生长、发育和应对各种非生物和生物胁迫的必需大量营养素，因为它在许多含硫化合物的生物合成中发挥着关键作用。硫酸盐只占土壤 S 拉力的一小部分，它是植物根部可以通过依赖 H+ 的共转运过程（即硫酸盐转运蛋白）吸收和动员的唯一形式。与其他有机结合形式的硫不同，硫酸盐由于易溶于水，通常会从土壤中浸出，从而降低了植物对其的利用率。尽管我们对植物硫酸盐转运蛋白的了解在过去几十年中显着增长，但对于丛枝菌根相互作用对硫吸收的影响仍然知之甚少。对植物部位的碳、氮和硫进行测量，并对编码假定的硫酸苜蓿转运蛋白 (MtSULTR) 的基因进行表达分析，以更好地了解菌根相互作用对不同硫酸盐浓度下根内球囊霉定殖的蒺藜苜蓿植物的有益影响。菌根化显着促进植物生长和硫含量，表明硫酸盐吸收增加。计算机分析允许识别八个假定的 MtSULTR，它们在系统发育上分布在四个硫酸盐转运蛋白组上。一些假定的 MtSULTR 在根和叶中的转录存在差异，并受到硫酸盐浓度的影响，而其他的 MtSULTR 则更具组成型转录。菌根诱导型和抑制型 MtSULTR 转录本的鉴定有助于阐明菌根相互作用在硫酸盐吸收中的作用。
Sulphur is an essential macronutrient for plant growth, development and response to various abiotic and biotic stresses due to its key role in the biosynthesis of many S-containing compounds. Sulphate represents a very small portion of soil S pull and it is the only form that plant roots can uptake and mobilize through H+-dependent co-transport processes implying sulphate transporters. Unlike the other organically bound forms of S, sulphate is normally leached from soils due to its solubility in water, thus reducing its availability to plants. Although our knowledge of plant sulphate transporters has been growing significantly in the past decades, little is still known about the effect of the arbuscular mycorrhiza interaction on sulphur uptake. Carbon, nitrogen and sulphur measurements in plant parts and expression analysis of genes encoding putative Medicago sulphate transporters (MtSULTRs) were performed to better understand the beneficial effects of mycorrhizal interaction on Medicago truncatula plants colonized by Glomus intraradices at different sulphate concentrations. Mycorrhization significantly promoted plant growth and sulphur content, suggesting increased sulphate absorption. In silico analyses allowed identifying eight putative MtSULTRs phylogenetically distributed over the four sulphate transporter groups. Some putative MtSULTRs were transcribed differentially in roots and leaves and affected by sulphate concentration, while others were more constitutively transcribed. Mycorrhizal-inducible and -repressed MtSULTRs transcripts were identified allowing to shed light on the role of mycorrhizal interaction in sulphate uptake.