The Self-Identity Protein IdsD Is Communicated between Cells in Swarming Proteus mirabilis Colonies
The Self-Identity Protein IdsD Is Communicated between Cells in Swarming Proteus mirabilis Colonies
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自我认同蛋白 IdsD 在奇异变形杆菌菌落中的细胞之间进行通讯
DOI：
10.1128/jb.00402-16
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发表时间：
2016
影响因子：
3.2
通讯作者：
Karine A. Gibbs
中科院分区：
文献类型：
--
作者：
C. Saak;Karine A. Gibbs
摘要奇异变形杆菌是一种社会性细菌，能够识别自我（亲属）和非自我。由于单个细胞的集体运动性，这种细菌的群集菌落在表面上向外扩展到厘米级的距离。遗传上不同的种群的殖民地保持分离，而相同种群的殖民地则合并。Ids蛋白质是这种识别行为所必需的。这些蛋白质中的两种，IdsD和IdsE，编码每个菌株的身份信息。这两种蛋白质在体外以等位基因限制性方式结合。IdsD-IdsE结合与群体的合并相关，而缺乏结合与群体的分离相关。关键问题仍然是关于IdsD和IdsE的体内相互作用，具体地说，IdsD和IdsE是否在单个细胞内结合，或者IdsD-IdsE相互作用是否发生在相邻细胞之间，如果是这样，这两种蛋白质中的哪一种被交换。在这里，我们证明，IDSD必须起源于另一个细胞来沟通的身份，这nonresident IDSD与IDSE居民在受体细胞相互作用。此外，我们表明，受体细胞中未结合的IdsD不会导致细胞死亡，而是似乎有助于限制群集集落的扩张半径。我们的结论是，P. mirabilis通信IdsD相邻细胞之间的非致命的亲属识别，这表明，Ids蛋白构成了一种类型的细胞间通信。我们证明了奇异变形杆菌中自我（亲属）与非自我的识别需要一种身份编码蛋白的细胞-细胞通信，该蛋白从一个细胞输出并被另一个细胞接收。我们进一步表明，这种细胞间的交流影响群体的殖民地扩张以非致命的方式，这增加了社会沟通的潜在群体相关的监管因素。
ABSTRACT Proteus mirabilis is a social bacterium that is capable of self (kin) versus nonself recognition. Swarming colonies of this bacterium expand outward on surfaces to centimeter-scale distances due to the collective motility of individual cells. Colonies of genetically distinct populations remain separate, while those of identical populations merge. Ids proteins are essential for this recognition behavior. Two of these proteins, IdsD and IdsE, encode identity information for each strain. These two proteins bind in vitro in an allele-restrictive manner. IdsD-IdsE binding is correlated with the merging of populations, whereas a lack of binding is correlated with the separation of populations. Key questions remained about the in vivo interactions of IdsD and IdsE, specifically, whether IdsD and IdsE bind within single cells or whether IdsD-IdsE interactions occur across neighboring cells and, if so, which of the two proteins is exchanged. Here we demonstrate that IdsD must originate from another cell to communicate identity and that this nonresident IdsD interacts with IdsE resident in the recipient cell. Furthermore, we show that unbound IdsD in recipient cells does not cause cell death and instead appears to contribute to a restriction in the expansion radius of the swarming colony. We conclude that P. mirabilis communicates IdsD between neighboring cells for nonlethal kin recognition, which suggests that the Ids proteins constitute a type of cell-cell communication. IMPORTANCE We demonstrate that self (kin) versus nonself recognition in P. mirabilis entails the cell-cell communication of an identity-encoding protein that is exported from one cell and received by another. We further show that this intercellular exchange affects swarm colony expansion in a nonlethal manner, which adds social communication to the list of potential swarm-related regulatory factors.
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影响因子：
3.6
作者：
Kapitein, Nicole;Boenemann, Gabriele;Mogk, Axel
通讯作者：
Mogk, Axel
影响因子：
10.5
作者：
Diner, Elie J.;Beck, Christina M.;Hayes, Christopher S.
通讯作者：
Hayes, Christopher S.
影响因子：
30.3
作者：
Hood RD;Singh P;Hsu F;Güvener T;Carl MA;Trinidad RR;Silverman JM;Ohlson BB;Hicks KG;Plemel RL;Li M;Schwarz S;Wang WY;Merz AJ;Goodlett DR;Mougous JD
通讯作者：
Mougous JD
影响因子：
4.4
作者：
Charles, C. J.;Rout, S. P.;Humphreys, P. N.
通讯作者：
Humphreys, P. N.