Determination of Cut-Off Titers and Agreement Between Immunofluorescence and Immunoblotting Methods for Detecting Antinuclear Antibodies in Children
Determination of Cut-Off Titers and Agreement Between Immunofluorescence and Immunoblotting Methods for Detecting Antinuclear Antibodies in Children
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DOI：
10.1002/jcla.20391
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发表时间：
2010-01-01
影响因子：
2.7
通讯作者：
Kutukculer, N.
中科院分区：
文献类型：
--
作者：
Aksu, G.;Gulez, N.;Kutukculer, N.
检测抗核抗体（ANA）是怀疑自身免疫性结缔组织疾病患者的诊断辅助手段，目前使用多种检测方法。本研究的目的是分析ANA免疫荧光（IF）和免疫印迹（IB）方法之间的一致性，并在实验室环境中确定儿童受试者的截止值。我们对729份血清样本进行了ANA、IF和IB分析。结果通过chi(2)检验进行评估，并使用kappa指数进行一致性评估。与IB测试相关的1:40-1:100 ANA IF截止滴度的频率支持1:100开始稀释的想法，应该推荐在儿童受试者中进行ANA IF方法和抗原特异性免疫印迹测试，特别是对于一些ANA IF阴性样本。两种方法之间的一致性，特别是同质，颗粒和核仁ANA IF模式，具有统计学意义。j .中国。实验室。《中国科学》，2010,24:230-236。(C) 2010 Wiley-Liss, Inc。
Detection of antinuclear antibodies (ANA) is a diagnostic adjunct in patients with suspected autoimmune connective tissue diseases, and various detection methods are in use. The aim of this study was to analyze the agreement between the ANA immunoflourescence (IF) and immunoblotting (IB) methods and determine cut-off for children subjects in a laboratory setting. We evaluated 729 serum samples that were analyzed by both ANA IF and IB. The results were evaluated by chi(2) test and, for agreement, kappa index was used. Frequencies determined for both 1:40-1:100 cut-off titers of ANA IF in relation to IB testing supported the idea that 1:100 starting dilution should be recommended in children subjects for ANA IF method and antigen specific immunoblot testing was needed, especially for some of the ANA IF negative samples. Agreement between the two methods, especially with homogenous, granular, and nucleolar ANA IF patterns, was statistically significant. J. Clin. Lab. Anal. 24:230-236, 2010. (C) 2010 Wiley-Liss, Inc.