Viral load, physical status, and E6/E7 mRNA expression of human papillomavirus in head and neck squamous cell carcinoma
Viral load, physical status, and E6/E7 mRNA expression of human papillomavirus in head and neck squamous cell carcinoma
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DOI：
10.1002/hed.23034
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发表时间：
2013-06-01
影响因子：
2.9
通讯作者：
Suzuki, Mikio
中科院分区：
文献类型：
--
作者：
Deng, Zeyi;Hasegawa, Masahiro;Suzuki, Mikio
本研究的目的是前瞻性地确定不同类型的头颈部鳞状细胞癌（HNSCC）中人乳头瘤病毒（HPV）载量和身体状况。方法采用聚合酶链反应（PCR）技术检测184例HNSCC患者HPV DNA、E6/E7 mRNA表达、病毒载量及体质状况。结果54例HNSCC中HPV基因组阳性率为29.3%，其中扁桃体癌阳性率为69.6%。与非口咽部HNSCC相比，口咽部HNSCC的病毒载量相对较高，尤其是扁桃体癌。75.6%的HPV-16阳性标本中E6/E7 mRNA表达为整合或混合状态，但仅有27.5%的HPV-16阳性标本中E6/E7 mRNA表达阳性。高HPV-16载量与E6/E7 mRNA表达显著相关。结论低病毒载量HNSCC患者E6/E7 mRNA表达水平较低，即使整合到宿主基因组中，E6/E7 mRNA表达水平也较低。在不同类型的HNSCC中，扁桃体癌与HPV显著相关。(c)2012 Wiley Periodicals，Inc.头颈部35：800-808，2013
Background The purpose of this study was to determine prospectively both human papillomavirus (HPV) load and physical status in different types of head and neck squamous cell carcinoma (HNSCC). Methods HPV DNA, E6/E7 mRNA expression, viral load, and physical status of 184 patients with HNSCC were examined simultaneously by polymerase chain reaction (PCR)-based methods. Results The HPV genome was detected in 54 HNSCC samples (29.3%), particularly in tonsillar carcinomas (69.6%). Compared with nonoropharyngeal HNSCC, oropharyngeal carcinoma harbored a relatively higher viral load, especially in tonsillar carcinoma. Although integrated or mixed status was observed in 75.6% of HPV-16positive samples, E6/E7 mRNA transcripts were detected in only 27.5% of HPV DNA-positive cases. High HPV-16 load correlated significantly with E6/E7 mRNA expression. Conclusion E6/E7 mRNA expression in patients with HNSCC with low viral load remains low even in cases of integration to the host genome. Tonsillar carcinomas were significantly associated with HPV among various types of HNSCC. (c) 2012 Wiley Periodicals, Inc. Head Neck 35: 800-808, 2013