Bryan Krantz: From folding to unfolding proteins. Interview by Liz Savage.
Bryan Krantz: From folding to unfolding proteins. Interview by Liz Savage.
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Bryan Krantz：从折叠到展开蛋白质。
DOI：
10.1083/jcb.1845pi
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Krantz,Bryan
中科院分区：
文献类型：
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作者：
Krantz,Bryan
大多数人在处理炭疽毒素之前可能会三思而后行，更不用说花几年的时间和它打交道了。但Krantz看到了这种毒素的潜力，它可以揭示蛋白质在通过细胞膜通道之前是如何展开的——这一过程被称为易位。炭疽毒素是由炭疽芽孢杆菌分泌的，它的三种蛋白质成分——保护性抗原、致死因子和水肿因子在靶细胞表面聚集。保护性抗原将自身插入细胞膜，并创建一个转位酶通道，允许其他两种蛋白质进入细胞。Krantz现在在加州大学伯克利分校经营着自己的实验室，他正在利用这种毒素系统来研究易位的生物物理机制。他相信这种传递毒素的方法也可能被其他病原体使用。
Most people might think twice before handling anthrax toxin, let alone spending years working with it. But Krantz saw the potential of this toxin for revealing how proteins unfold before they pass through cell membrane channels—a process known as translocation. Anthrax toxin is secreted from the bacteria Bacillus anthracis, and its three protein components—protective antigen, lethal factor, and edema factor—assemble on the surfaces of target cells. The protective antigen inserts itself into a cell membrane and creates a translocase channel, which allows the other two proteins to enter into the cell. Krantz, who now runs his own laboratory at the University of California, Berkeley, is using this toxin system to study the biophysical mechanisms of translocation (4). He believes this method of toxin delivery might also be used by other pathogens.