Immunohistochemical comparison of α- and β-synuclein in adult rat central nervous system
Immunohistochemical comparison of α- and β-synuclein in adult rat central nervous system
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DOI：
10.1016/s0006-8993(02)02643-4
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发表时间：
2002-06-21
期刊：
影响因子：
2.9
通讯作者：
Wakabayashi, K
中科院分区：
文献类型：
--
作者：
Mori, F;Tanji, K;Wakabayashi, K
我们研究了α-和β-突触核蛋白在成年大鼠中枢神经系统的免疫组织化学定位，并与突触前标志物突触素(SYN)的分布进行了比较。反SYN。Alpas和Betas抗体呈点状免疫标记神经纤维束，神经元胞浆内未见SYN免疫反应。相反，抗Alpas抗体和Betas抗体均对神经元胞浆和胞核进行了不同程度的免疫染色，这取决于大脑区域。阿尔法阳性神经元出现在嗅球，即大脑新皮质的深层。在黑质和桥核中，黑质神经元的免疫反应最强。PS阳性神经元广泛分布于中枢神经系统，嗅球颗粒细胞层PS阳性神经元占50%以上。大脑皮层第四层、海马区CA3、基底节和丘脑网状核。此外，不同的Se蛋白在中枢神经系统某些区域的神经纤维束中的免疫反应分布模式也存在差异。这些发现表明，虽然US和PS集中在突触前终末，但大量的这些蛋白也存在于神经细胞膜。此外，AlpHas阳性神经元的分布类似于AlpHas转基因动物和人类α-突触核病的神经元内包涵体。(C)2002 Elsevier Science B.V.保留所有权利。
We investigated the immunohistochemical localization of alpha- and beta-synuclein (alphaS and betaS) in the adult rat CNS and compared the distribution of these proteins with that of synaptophysin (SYN), a well-established presynaptic marker. Anti-SYN. alphaS and betaS antibodies immunolabeled the neuropil in a punctate pattern, No SYN immunoreactivity was found in the neuronal cytoplasm. In contrast, both anti-alphaS and betaS immunostained the neuronal cytoplasm and nucleus to various extents depending on the brain region. alphaS-positive neurons were found in the olfactory bulb, deeper layers of the cerebral neocortex. substantia nigra and pontine nucleus, Immunoreactivity was most intense in neurons of the substantia nigra. PS-positive neurons were more widely distributed throughout the CNS: more than 50% of neurons were positive for PS in the granular cell layer of the olfactory bulb. layer IV of the cerebral cortex, CA3 of the hippocampus, basal ganglia and thalamic reticular nuclei. Moreover, there were difference,, among the, se proteins in the distribution pattern of immunoreactivity in the neuropil in some CNS regions. These findings suggest that although US and PS are concentrated in presynaptic terminals, significant amounts of these proteins are also present in the neuronal perikarya. Furthermore, the distribution of alphaS-positive neurons is similar to that of intraneuronal inclusions in alphaS transgenic animals as well as in human alpha-synucleinopathies. (C) 2002 Elsevier Science B.V. All rights reserved.