Distribution of syndecans 1-4 within the anterior segment of the human eye: expression of a variant syndecan-3 and matrix-associated syndecan-2
Distribution of syndecans 1-4 within the anterior segment of the human eye: expression of a variant syndecan-3 and matrix-associated syndecan-2
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DOI：
10.1016/j.exer.2004.02.010
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发表时间：
2004-07-01
影响因子：
3.4
通讯作者：
Peters, DM
中科院分区：
文献类型：
--
作者：
Filla, MS;David, G;Peters, DM
肌动球蛋白网络的控制在调节通过眼前段的房水运动中起作用。控制其活性的受体尚不清楚。在这项研究中，我们表明，所有四个成员的syndecan家庭，可以调节肌动球蛋白网络，是目前在眼前段。在人眼前节的切片和人小梁网（HTM）、Schlemm管（HSC）和来自眼前节的睫状肌（HCM）细胞的培养物中，syndecans-3和-4是主要的家族成员。它们广泛分布于眼前段。Syndecan-3在眼前节是一种新的，最近描述的变异55 kDa的形式。在原位和所有三种培养物中也观察到低水平的多配体蛋白聚糖-1和-2。它们的表达比多配体蛋白聚糖-3和-4所观察到的更弱且更局部化。HCM培养物中syndecan-1的染色是可变的。在HTM和HSC培养物中，多配体蛋白聚糖-2还与纤连蛋白、层粘连蛋白和IV型胶原共分布，表明其脱落并与细胞外基质相关。蛋白质印迹支持这一观点，并显示syndecan-2胞外域的裂解物从眼前节。(C)2004爱思唯尔有限公司保留所有权利。
Control of the actomyosin network plays a role in regulating the movement of aqueous humor through the anterior segment of the eye. Receptors that could control its activity are unknown. In this study, we show that all four members of the syndecan family, which can regulate the actomyosin network, are present within the anterior segment. In both sections of human anterior segments and Cultures of human trabecular meshwork (HTM), Schlemm's canal (HSC) and the ciliary muscle (HCM) cells from the anterior segment, syndecans-3 and -4 were the predominant family members. They were widely distributed throughout the anterior segment. Syndecan-3 within the anterior segment was a novel, recently described variant 55 kDa form. Low levels of syndecans-1 and -2 were also observed in situ and in all three cultures. Their expression was weaker and more localized than that observed for syndecans-3 and -4. Staining for syndecan-1 in HCM cultures was variable. In HTM and HSC cultures, syndecan-2 also co-distributed with fibronectin, laminin and type IV collagen suggesting that it was shed and associated with the extracellular matrix. Western blots supported this idea and showed syndecan-2 ectodomains in lysates from anterior segments. (C) 2004 Elsevier Ltd. All rights reserved.